Screening of Diuretic Activity Fractions and Chemical Compositions in Rattan of

GUO Lin-xin; MA Yang-min; LI Meng-yun; QIAO Ke; WANG Jian

doi:10.13422/j.cnki.syfjx.2017030066

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Screening of Diuretic Activity Fractions and Chemical Compositions in Rattan of

更新时间：2024-01-04

- Screening of Diuretic Activity Fractions and Chemical Compositions in Rattan of
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 3, Pages: 66-70(2017)
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- DOI：10.13422/j.cnki.syfjx.2017030066
  CLC： R284.1
- Published：2017
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GUO Lin-xin, MA Yang-min, LI Meng-yun, et al. Screening of Diuretic Activity Fractions and Chemical Compositions in Rattan of [J]. Chinese journal of experimental traditional medical formulae, 2017, 23(3): 66-70.
DOI：

GUO Lin-xin, MA Yang-min, LI Meng-yun, et al. Screening of Diuretic Activity Fractions and Chemical Compositions in Rattan of [J]. Chinese journal of experimental traditional medical formulae, 2017, 23(3): 66-70. DOI： 10.13422/j.cnki.syfjx.2017030066.

摘要

目的：筛选三叶木通利尿活性部位并对其化学成分进行研究。方法：采用大鼠代谢笼对三叶木通藤茎95%乙醇提取物的石油醚、乙酸乙酯、正丁醇萃取物和水溶部分的利尿作用进行测试。以硅胶柱色谱等方法对利尿作用显著的部位进行分离纯化，通过NMR，MS等方法并结合文献数据鉴定化合物结构。结果：正丁醇萃取物能显著增加大鼠尿量（P<0.01），其余各部位利尿作用不明显。在该部位分离得到9个化合物，分别鉴定为D-半乳糖（1），丁香酸-4-O-α-L-鼠李吡喃糖苷（2），常春藤皂苷元（3），肌醇（4），甘露醇（5），香草酸葡萄糖苷（6），丁香酸葡萄糖苷（7），常春藤皂苷元-3-O-α-L-阿拉伯吡喃糖苷（8）和β-谷甾醇-3-O-β-D-葡萄糖苷（9）。结论：三叶木通提取物的正丁醇萃取部位具有显著的利尿作用，其主要化学成分为苷类化合物。

Abstract

Objective: To screen the diuretic active fractions from rattan of Akebia trifoliate and study its chemical constituents. Method: The 95%ethanol extract of rattan of A. trifoliate

which were extracted with petroleum ether

ethyl acetate

butanol and water dissolving fractions

were investigated through metabolic cages experiments. The compounds of active fractions were isolated and purified by silica gel column chromatography and other methods

and their structures were identified by NMR and MS combined with literature data. Result: The butanol extract fraction can significantly increase the urine volume of rats(P<0.01)

but the diuretic effect of other fractions was not obvious. Nine compounds were isolated from this diuresis fraction

and their structures were identified as D-galactose (1)

syringicacid-4-O-α-L-rhamnopyranoside (2)

hederagenin(3)

inositol(4)

mannitol (5)

vanillic acid-β-glucoside (6)

glucosyringic acid (7)

hederagenin-3-O-α-L-arabinopyranoside (8) and β-sitosterol-3-O-β-D-glycoside (9). Conclusion: The butanol extract fraction had significant diuretic function

and glycosides were the main chemical composition of this fraction.

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