Objective: To establish a method for simultaneous contents determination of 4 flavonoids including quercetin

kaempferide

pinocembrin

and galangin as well as alkaloids piperine in Uighur medicine compound Xiaoaifei by using HPLC. Method: Separation was performed on Hypersil BDS C18(4.6 mm×250 mm

5 μm) column with acetonitrile (A)-1% phosphoric acid (B)-methanol (C) (0-12 min

28%A:52%B:20%C; 12-13 min

28%-30%A

52%-50%B

20%C; 13-45 min

30%A:50%B:20%C) as the mobile phase for gradient elution at a flow rate of 1.0 mL·min-1. The detection wavelength was set at 280 nm; the column temperature was 30℃

and the sample size was 20 μL. Result: Y=357 314X-88 066 (r=0.999 9)

Y=442 853X-74 204 (r=0.999 9)

Y=647 926X-327 436 (r=0.999 9)

Y=471 672X-256 408 (r=0.999 9)

and Y=290 000X-391 906 (r=0.999 9)

respectively in the calibration curves of quercetin

kaempferide

pinocembrin

galangin and piperine

showing good linear relationship. The mean recoveries (n=9) of them were 98.4% (RSD 1.6%)

98.9% (RSD 1.7%)

101.3% (RSD 2.2%)

98.6% (RSD 2.1%) and 98.6% (RSD 1.8%)

respectively. Conclusion: The developed method is simple

accurate

reliable

and helpful to control the quality of Uighur medicine compound Xiaoaifei.