Objective: To study the effect of Ferulae Resina ethyl acetate extract on the migration and invasion of human colon adenocarcinoma cell line Caco-2 and its relevant mechanism. Method: With Caco-2 cells as the research object

6.25

12.5 mg·L-1 of Ferulae Resina ethyl acetate extract and cisplatin were respectively applied in human colon adenocarcinoma Caco-2 cells in the logarithmic phase of growth. A blank control group was set up. Scratch healing method and transwell chamber were used to observe 0-48 h Caco-2 cells migration in each group and the number of Caco-2 cell passing through the matrix membrane in 72 h in each group

and calculate Caco-2 cell migration rate and invasion rate at each time point. Gene and protein expressions of phosphatase and tensin homolog (PTEN)

serine/threonine (Akt)

mammalina target of rapamgcin (mTOR)

and protein in Caco-2 cells were detected by Real-time PCR and Western blot. Result: Ferulae Resina and cisplatin groups showed slower cells healing degrees at 24 h and 48 h compared with the blank group (P<0.05)

at 72 h

the number of cells passing through the matrix membrane was significantly less than that in blank group (P<0.01). In each dose group

PTEN gene expression levels were significantly higher

and Akt

mTOR gene expression levels were significantly lower (P<0.05). Conclusion: Ferulae Resina ethyl acetate extract can significantly inhibit the migration and invasion of human colon adenocarcinoma Caco-2 cells

which may be correlated with increase in PTEN gene expression.