Hypolipidemic Effect of Zexietang and Correlation with Intestinal Microflora

XU Xiao-mei; LIN Wen-jin; ZHANG Ya-min; XU Rong-qing

doi:10.13422/j.cnki.syfjx.2017030116

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Hypolipidemic Effect of Zexietang and Correlation with Intestinal Microflora

更新时间：2024-01-04

- Hypolipidemic Effect of Zexietang and Correlation with Intestinal Microflora
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 3, Pages: 116-121(2017)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2017030116
  CLC： R285.5
- Published：2017
- 稿件说明：
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XU Xiao-mei, LIN Wen-jin, ZHANG Ya-min, et al. Hypolipidemic Effect of Zexietang and Correlation with Intestinal Microflora[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(3): 116-121.
DOI：

XU Xiao-mei, LIN Wen-jin, ZHANG Ya-min, et al. Hypolipidemic Effect of Zexietang and Correlation with Intestinal Microflora[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(3): 116-121. DOI： 10.13422/j.cnki.syfjx.2017030116.

摘要

目的：研究泽泻汤抗大鼠高脂血症的作用与肠道微生态变化的相关性，并通过高通量测序技术测定大鼠肠道微生物变化。方法：将40只健康雄性SD大鼠随机分为正常组，高脂模型组，辛伐他汀预处理组（阳性药组，2.1 mg·kg-1·d-1），泽泻汤预处理组（2.2 g·kg-1·d-1），每组10只，造模4周后分别灌胃（ig）给予相应的药物，连续4周，正常组及高脂模型组ig给予等量的生理盐水。检测血清总胆固醇（TC），总甘油三酯（TG），低密度脂蛋白胆固醇（LDL-C）及高密度脂蛋白胆固醇（HDL-C）水平；伊红-苏木素（HE）检测肝脏组织形态学变化；提取肠道微生物总DNA，检测肠道微生物变化。结果：高脂模型组大鼠TC，TG，LDL-C水平明显高于正常组（P<0.05），给药4周后，与模型组比较，给药组TC，TG，LDL-C水平均降低（P<0.05）；组织形态学检测显示模型组大鼠肝脏颜色呈粉白色，组织肿大，表面粗糙，相对正常组弹性较差；与模型组大鼠比较，泽泻汤组与辛伐他汀组大鼠肝脏颜色、弹性均明显好转；16S rDNA 基因序列分析显示，治疗组大鼠肠道菌群多样性及丰富度明显增加，肠道正常菌群的数目在一定程度上得到恢复，肠道菌群失调得到改善。结论：泽泻汤可通过肠道菌群这一靶标，进行脂质代谢的调节，从而发挥抗高脂血症的作用。

Abstract

Objective: To study the correlation between the effect of Zexietang on hyperlipidemic rats and the changes in intestinal microflora. Method: Totally 40 healthy male SD rats were randomly divided into normal control group (group C)

high fat diet group (group M)

high fat diet plus simvastatin pretreatment group (positive control group

group Y

2.1 mg·kg-1·d-1) and high fat diet plus Zexietang pretreatment group (group Z

2.2 g·kg-1·d-1)

with 10 in each group. After modeling for 4 weeks

both of group Y and group Z were given the corresponding drugs by gavage once a day. Group C and group M were given the same volume of normal saline. After administration for 4 weeks

the levels of serum total cholesterol(TC)

total triglyceride(TG)

low density lipoprotein cholesterol(LDL-C) and high density lipoprotein cholesterol(HDL-C) were detected. HE was performed to detect the histological changes of livers. Total DNA was extracted from the intestinal tract

and the changes of intestinal microflora were detected. Result: The levels of TC

LDL-C in group M were higher than that of group C (P<0.05). After pretreatment with Zexietang for 4 weeks

the hyperlipidemic rats in group Z and group Y displayed reductions in levels of TC

TG and LDL-C compared with the model group (P<0.05). According to the histomorphological test

rats in the model group showed liver in light pink color

with tissue swelling

surface roughness and lower elasticity than the normal group; Compared with the model group

group Z and group Y showed significant improvement in liver color and elasticity. Furthermore

the intestinal bacterial 16S rDNA high-throughput sequencing analysis results showed that in the Zexietang pretreatment group

the structural diversity and richness of intestinal flora of the rats were obviously increased. Moreover

the number of normal intestinal flora was recovered to a certain extent

and the disorder of intestinal flora was alleviated. Conclusion: Zexietang can effectively regulate the disorder of lipid profiles metabolism in hyperlipidemic rats by regulating the target of intestinal flora

so as to play the role of anti-hyperlipidemia.

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