Protective Effect of Buyang Huanwu Tang on Human Umbilical Vascular Endothelial Cells Injured by Oxidized Low Density Lipoprotein

JING Wen; WU Hao-xin; CHAI Yi

doi:10.13422/j.cnki.syfjx.2017030128

您当前的位置：

首页 >

文章列表页 >

Protective Effect of Buyang Huanwu Tang on Human Umbilical Vascular Endothelial Cells Injured by Oxidized Low Density Lipoprotein

更新时间：2024-01-04

- Protective Effect of Buyang Huanwu Tang on Human Umbilical Vascular Endothelial Cells Injured by Oxidized Low Density Lipoprotein
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 3, Pages: 128-133(2017)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2017030128
  CLC： R285
- Published：2017
- 稿件说明：
移动端阅览
JING Wen, WU Hao-xin, CHAI Yi. Protective Effect of Buyang Huanwu Tang on Human Umbilical Vascular Endothelial Cells Injured by Oxidized Low Density Lipoprotein[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(3): 128-133.
DOI：

JING Wen, WU Hao-xin, CHAI Yi. Protective Effect of Buyang Huanwu Tang on Human Umbilical Vascular Endothelial Cells Injured by Oxidized Low Density Lipoprotein[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(3): 128-133. DOI： 10.13422/j.cnki.syfjx.2017030128.

摘要

目的：观察加味补阳还五汤（BHT）含药血清和含药血浆对氧化型低密度脂蛋白（ox-LDL）损伤血管内皮细胞（VEC）的保护作用及机制，同时比较加味BHT含药血清和含药血浆对VEC生成影响的差异。方法：制备加味BHT含药血清和含药血浆，体外培养人脐静脉血管内皮细胞（HUVECs），采用ox-LDL造模，通过噻唑蓝（MTT）法观察细胞活力和Hoechst33258染色法、流式细胞术观察细胞凋亡率，并通过测定一氧化氮（NO），丙二醛（MDA）含量和超氧化物歧化酶（SOD），谷胱甘肽过氧化物酶（GPx）活力，探讨加味BHT含药血清和含药血浆对ox-LDL损伤VEC的保护作用及含药血清和含药血浆之间的差异。结果：与空白组比较，模型组细胞死亡和凋亡率明显升高（P<0.05），和模型组比较，加味BHT含药血清组和含药血浆组能显著降低ox-LDL造成的HUVECs的凋亡和死亡率（P<0.05），细胞活性显著增强（P<0.05），NO含量，SOD和GPx活力显著提高，MDA含量均显著下降（P<0.05）。此外，含药血清组与含药血浆组比较细胞凋亡和死亡率更低（P<0.05），含药血浆组与含药血清组比较产生NO含量明显增加（P<0.05）。结论：加味BHT含药血清和含药血浆对于ox-LDL损伤的VEC具有一定保护作用，其机制可能与改善内皮细胞功能、抗氧化损伤、抑制细胞凋亡有关。在一定条件下对于加味BHT含药血浆保护VEC的作用强于含药血清。

Abstract

Objective: To investigate the protective effects and mechanism of Buyang Huanwu Tang (BHT) on vascular endothelial cells (VEC) injured by oxidized low density lipoprotein (ox-LDL)

and compare the effect between drug-containing serum and drug-containing plasma on VEC. Method: The modified BHT-containing serum and plasma were prepared. Human umbilical vascular endothelial cells (HUVECs) were treated with serum and plasma containing modified BHT and incubated with ox-LDL for additional 24 hours for ox-LDL modeling. The cell viability was observed by MTT assay

and cells apoptosis rate was observed by using Hoechst 33258 staining and flow cytometry; meanwhile the levels of nitric oxide (NO)

malondialdehyde (MDA)

and the activity of superoxidedismutase (SOD)

glutathione peroxidase (GPx) were determined to explore the difference in the protective effect between modified BHT-containing serum and plasma on injured VEC. Result: As compared with the blank control group

the percentage of cells death and apoptosis was significantly higher in the model group (P<0.05). As compared with the model group

the percentage of cells death and apoptosis was significantly reduced in modified BHT-containing serum group and plasma group (P<0.05); cell viability was significantly increased(P<0.05); the levels of NO

SOD and GPx were all significantly increased

the level of MDA was significantly reduced (P<0.05). Besides

the percentage of cells death and apoptosis was lower in modified BHT-containing serum group than that in plasma group (P<0.05); however

the level of NO in modified BHT-containing plasma group was significantly higher than that in serum group (P<0.05). Conclusion: The serum and plasma containing modified BHT could prevent VEC from oxidative injury induced by ox-LDL

and the mechanism may be associated with improving the function of endothelial cells

anti-oxidative damage and inhibiting cell apoptosis. The protective effect of modified BHT-containing plasma on VEC was more stronger than the modified BHT-containing serum under certain conditions.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Mechanism of Yiyuan Qiwei Pills in Treatment of Diabetes Mellitus-induced Erectile Dysfunction

Protective Effect of Danggui Buxue Tang on Impaired Functional Activity of Endothelial Cells Exposed to Low Flow Shear Stress

Effect of Exogenous Nitric Oxide on Plant Hormones and Enzyme Content of Acanthopanax senticosus Seeds

Periodic Observation for NO Level and NOS Activity in Serum and Brain Tissue of Rats Damaged by Cerberal lschemic Reperfusion with Naoluotong

Nitric Oxide Supersensitivity induced by hyposerotonin and the Pharmacological Effect of Dachuanxiong Pill

Related Author

Ding WANG

Shaofeng ZHANG

Xue JIANG

Xiang LYU

Taiping ZHU

Jiakun LIN

Zhen QIN

Zheng-xin WEI

Related Institution

Shijiazhuang Medical College

Baixiang Country Central Hospital

Pingxiang Hospital of Traditional Chinese Medicine

School of Basic Medicine, Guizhou Medical University

School of Extended Education, Guangzhou University of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰