Protective Effect of Active Components Compatibility of  on Immunological Liver Injury Induced by Concanavalin A in Mice

LIU Mei-lin; DONG Jia-yue; LI Yang; AN Tai-yong; ZHAO Lu-li; ZHANG Mei

doi:10.13422/j.cnki.syfjx.2017030146

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Protective Effect of Active Components Compatibility of on Immunological Liver Injury Induced by Concanavalin A in Mice

更新时间：2024-01-04

- Protective Effect of Active Components Compatibility of on Immunological Liver Injury Induced by Concanavalin A in Mice
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 3, Pages: 146-150(2017)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2017030146
  CLC： R285.5
- Published：2017
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LIU Mei-lin, DONG Jia-yue, LI Yang, et al. Protective Effect of Active Components Compatibility of on Immunological Liver Injury Induced by Concanavalin A in Mice[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(3): 146-150.
DOI：

LIU Mei-lin, DONG Jia-yue, LI Yang, et al. Protective Effect of Active Components Compatibility of on Immunological Liver Injury Induced by Concanavalin A in Mice[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(3): 146-150. DOI： 10.13422/j.cnki.syfjx.2017030146.

摘要

目的：探究波棱瓜子中活性成分乙酸乙酯提取物（HE）与脂肪油（HSO）配伍对刀豆蛋白A（ConA）诱导的免疫性肝损伤小鼠的保护作用及其机制。方法：将小鼠80只随机区组分为8组，即空白组，模型组，联苯双酯组（阳性组），HSO组，HE组，组分配伍1：1，1：2，2：1组，每组10只。治疗组分别灌胃（ig）给予波棱瓜子HE，HSO及二者不同剂量配伍药液连续8 d，采用尾静脉一次性注射ConA 30 mg·kg-1诱导造成小鼠免疫性肝损伤模型。造模8 h后采血，制备肝匀浆。计算小鼠的肝脾系数；酶联免疫法（ELISA）检测小鼠血清谷丙转氨酶（ALT），谷草转氨酶（AST），一氧化氮（NO）及肝脏匀浆中超氧化物歧化酶（SOD），丙二醛（MDA）等生化指标的活性及含量；同时对肝组织进行病理学检查。结果：波棱瓜子各治疗组均可降低免疫性肝损伤小鼠的肝脾脏器指数；降低小鼠肝损伤血清ALT，AST水平及NO的含量；增强肝匀浆中SOD活性，降低MDA的含量；减轻ConA对肝组织的病理损伤。其中有效组分HSO-HE 2：1配伍组各指标较模型组均有显著性差异，护肝作用最佳。结论：波棱瓜子HE与HSO配伍护肝作用优于单一组分，提示配伍时具有协同增效的作用，其保肝机制可能与其抗炎、抗氧化以及清除自由基有关。

Abstract

Objective: To explore the protective effect and mechanism of active components compatibility of ethyl acetate extracts(HE) and fatty oil(HSO) of Herpetospermum caudigerum on immunologic liver injury induced by concanavalin A (ConA) in mice. Method: The 80 mice were randomly divided into 8 groups (n=10)

normal group

model group

biphenyl double ester group (positive control)

HSO group

HE group

HSO and HE of 1:1

1:2

2:1 ratio groups. After ig administration of ethyl acetate extracts

fatty oil and different compatibilities for 8 days in treatment group

the mice were injected with ConA (30 mg·kg-1) through tail vein to establish the model of immunological liver injury. Blood was collected after 8 hours modeling to prepare liver homogenate. Liver and spleen indexes were calculated; the contents of alanine aminotransferase (ALT)

aspartate aminotransferase (AST) and nitrogen monoxidum (NO) in serum

superoxide dismutase (SOD)

malondialdehyde (MDA) in liver homogenate were assayed by ELISA; and pathological morphological changes in liver cells were monitored by HE. Result: Ethyl acetate extracts

fatty oil and their combinations of H. caudigerum could decrease liver and indexes

the levels of ALT

AST

MDA and increased SOD activities. Meanwhile

the pathological damage in liver was relieved. As compared with the model group

thefatty oiland ethyl acetate extracts at 2:1 ratio group showed the best effect on immunological liver injury in mice

with significant differences in above indexes. Conclusion: Ethyl acetate extracts compatibility with oil can protect immunological liver injury in mice

and the effect was superior to that of single component. The mechanism may be associated with anti-inflammatory

anti-oxidation and scavenging oxygen free radical.

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