Objective: To discuss the effect of Massa Medicata Fermentata composed by fresh or dry Chinese medicines and prepared by different preparation methods on gastric emptying

intestine propulsion and regulating intestinal flora of dyspepsia mice. Method: Four groups of Massa Medicata Fermentata samples were prepared

the first group is the basic one composed of flour

rice bean and bitter almond

the fresh product groups (the basic group and fresh medicine juicing or decocting) and the dry product group (the basic group and dry medicine decocting).Mice were used to set up dyspepsia and gastrointestinal disorders model by ig 100% refine lard oil with dose of 0.4 mL·g-1 for 10 d.Experimental animals were divided into 6 groups (the blank group

the model group and four sample groups).Sample groups were given Massa Medicata Fermentata suspension by oral administration with 0.2 mL·g-1

the blank group and the model group were administrated equivalent distilled water

once a day and for seven consecutive days.Impact of sample groups on gastric emptying rate and intestine propulsion rate was observed.Flora condition in colonic contents was detected by polymerase chain reaction technique (PCR). Result: In the gastrointestinal dynamics experiment

compared with the model group

each drug administration group could promote the movement of gastric emptying and intestinal propulsion

but their levels were different.In the detection of intestinal flora

each drug administration group could raise significantly the growth of intestinal beneficial bacteria (such as lactobacillus

bifidobacterium and bacteroides) and their levels were different. Conclusion: Massa Medicata Fermentata composed of fresh Chinese medicines can promote stomach intestine movement significantly and regulate the intestinal flora

both experiments led to corresponding results together.Massa Medicata Fermentata composed of fresh Chinese medicines is better than dry ones.The principles of formulating prescription about Massa Medicata Fermentata is fresh medicine decocting for the best.The spleen function of Massa Medicata Fermentata is concerned with promoting gastrointestinal

adjusting the intestinal flora and promoting the growth of bacteria.