Objective: To compare the inhibition effect of raw and rice-wine products of Trigonellae Semen on α-glucosidase and α-amylase for screening of effective fraction with inhibiting activity.Then try to analyze its reaction kinetics. Method: Four different polar fractions

including petroleum ether fraction

ethyl acetate fraction

n-butanol fraction and water fraction

were extracted from 80% methanol extract of raw and rice-wine products of Trigonellae Semen.α-Glucosidase inhibitory model and α-amylase inhibition model in vitro were adopted to measure inhibition effect of different polar fractions on α-glucosidase and α-amylase.Then their inhibitory types were inferred by enzymatic dynamics and Lineweaver-Burk curve methods. Result: The water fraction from raw and rice-wine products of Trigonellae Semen had inhibition effect on α-glucosidase with non-competitive and reversible type

the rice-wine product of Trigonellae Semen was better than raw product.The ethyl acetate and n-butanol fraction from raw and rice-wine products of Trigonellae Semen had inhibition effect on α-amylase with non-competitive and reversible type

and the rice-wine product of Trigonellae Semen was better than raw product. Conclusion: The inhibition effect of Trigonellae Semen on α-glucosidase and α-amylase can be increased after being processed by rice-wine.The water fraction from rice-wine product has medicinal development values as a new α-glucosidase inhibitor

the ethyl acetate and n-butanol fraction from rice-wine product has medicinal development values as new α-amylase inhibitors.