Objective: To provide basis for utilization and development of germplasm resources of Rosae Laevigatae Fructus. Method: Totally 20 germplasms of Rosae Laevigatae Fructus were collected from Zhejiang

Chongqing

Hunan

Shanxi

Guangdong

Anhui and Jiangxi provinces of China. Subsequently

longitude and latitude

elevation and agronomic traits were recorded and measured

while polysaccharose contents were measured by phenol-sulfuric acid method. The data obtained were analyzed by SPSS 17.0 and DPS 9.05 software with ANOVA and UPGMA methods respectively. Result: There were significant differences in agronomic traits and polysaccharose contents among Rosae Laevigatae Fructus from different origins (P<0.01)

and polysaccharose content of germplasm in Zhejiang was relatively high. Meanwhile

there were variation rules between polysaccharose contents as well as agronomic traits and biotope factor. In particular

there were significantly positive relations between polysaccharose content as well as ratio of length and width of leaves

ratio of vertical and lateral diameter of fruit and longitude

with negative relations with lateral diameter of fruit

and low relations with weight of fruit and fruit fresh

and latitude. What's more

10 characters were simplified to 4 principle components

with the accumulative contribution rate up to 94.69%. Besides

20 germplasms were divided into 5 groups. Among them

the first group showed a higher ratio of length and width of leaves and ratio of vertical and lateral diameter of fruit

with a higher polysaccharose contents. The fourth and fifth groups showed a broad and thick leave area

low ratio of length and width of leaves

large fruit and higher ratio of vertical and lateral diameter of fruit. Conclusion: Five germplasms with superior agronomic traits and four germplasms with higher polysaccharose content have been selected

and could be used for breeding high-yield and high-quality Rosae Laevigatae Fructus.