Isolation and Identification of Secondary Metabolites from Endophytic  sp. in Astragali Radix

ZHANG Hong-chi; LIU Rui; LI Hui; AN Zhi-peng; ZHOU Feng

doi:10.13422/j.cnki.syfjx.2017040071

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Isolation and Identification of Secondary Metabolites from Endophytic sp. in Astragali Radix

更新时间：2024-01-04

- Isolation and Identification of Secondary Metabolites from Endophytic sp. in Astragali Radix
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 4, Pages: 71-75(2017)
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- DOI：10.13422/j.cnki.syfjx.2017040071
  CLC： S567.239;Q936
- Published：2017
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ZHANG Hong-chi, LIU Rui, LI Hui, et al. Isolation and Identification of Secondary Metabolites from Endophytic sp. in Astragali Radix[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(4): 71-75.
DOI：

ZHANG Hong-chi, LIU Rui, LI Hui, et al. Isolation and Identification of Secondary Metabolites from Endophytic sp. in Astragali Radix[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(4): 71-75. DOI： 10.13422/j.cnki.syfjx.2017040071.

摘要

目的：研究恒山黄芪内生真菌Rhizopus sp.的次生代谢产物的类别。方法：通过组织学的方法从恒山黄芪中分离到AR02，真菌的鉴定采用形态学法结合分子生物学法，内生真菌大量发酵采用固体培养的方法，次生代谢产物的分离采用硅胶柱色谱法，Sephadex LH-20凝胶色谱法结合HPLC进行分离纯化，分离到的代谢产物的化学结构通过理化分析测定结合光谱分析进行鉴定。结果：从黄芪根部分离到的内生真菌AR02被鉴定为根霉属Rhizopus sp.，从该菌株的固体发酵产物中分离得到7个化合物，经结构鉴定，分别为色胺酮（1），lawsaritol A（2），曲菌酸（3），trypacidin（4），monomethylsulochrin（5），emodin 8-O-methylether（6）和cannabifolactone A（7）。结论：化合物1~3，6和7均为首次从恒山黄芪内生真菌中分离得到，且黄芪内生真菌Rhizopus sp.的次生代谢产物呈现出多个类别，可以作为活性物质的新来源。

Abstract

Objective: To study on secondary metabolites of endophytic fungus Rhizopus sp. from Astragali Radix. Method: AR02 was isolated from Astragali Radix by the method of histology. The fungus was identified by morphological method combined with molecular biology method. Large-scale fermentation of endophytic fungi used solid culture method. The compounds were isolated by column chromatography

Sephadex LH-20

and HPLC. They were identified on the basis of physic-chemical constants and spectral analysis. Result: Endophytic fungi AR02 isolated Astragali Radix were identified as Rhizopus sp. Seven compounds were obtained and elucidated as tryptanthrin (1)

lawsaritol A (2)

kojic acid (3)

trypacidin (4)

monomethylsulochrin (5)

emodin 8-O-methylether (6) and cannabifolactone A (7). Conclusion: Compounds 1-3

6 and 7 were isolated from the endophytic fungi of Astragali Radix for the first time. The secondary metabolites of Rhizopus sp. appear in several categories and the endophytic fungus

Rhizopus sp. from Astragali Radix is a promising source for active substances.

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