GAO Li-na, JIA Run-qing, AI Ping, et al. Anti-HIV-1 Activity of Extracts from 6 Traditional Tibetan Medicines[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(4): 171-176.
DOI:
GAO Li-na, JIA Run-qing, AI Ping, et al. Anti-HIV-1 Activity of Extracts from 6 Traditional Tibetan Medicines[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(4): 171-176. DOI: 10.13422/j.cnki.syfjx.2017040171.
Anti-HIV-1 Activity of Extracts from 6 Traditional Tibetan Medicines
Objective: To evaluate the in vitro anti-HIV-1 activities and mechanism of extracts from 6 traditional Tibetan medicines:Corydalis conspersa
Fallopia aubertii
Anemone vitifolia
Clematis urophylla
Saxifraga melanocentra and C. hexapetala. Method: Cell counting kit(CCK-8) cell cytotoxicity test and HIV-1 single cycle infection experiments were used to detect the in vitro anti-HIV-1 effect and cytotoxicity of extracts. The surface plasma resonance(SPR) technology
protease
integrase and reverse transcriptase inhibition assay were used to identify the potential targets of these extracts. Result: The extracts from F. aubertii
A. vitifolia and S. melanocentra showed dose-dependent anti-HIV-1 effect with little cytotoxicity. Their half maximal inhibitory concentrations (IC50) were (6.47±0.78)
(11.97±1.09)
(11.7±0.79) mg·L-1
respectively. All the above three extracts inhibited integrase activity. Conclusion: F. aubertii
A. vitifolia and S. melanocentra extracts potently inhibited HIV-1 virus in vitro
possibly through down-regulating integrase activity.