Objective: To investigate cytotoxicity and cellular uptake of brucine-loaded solid lipid nanoparticles (B-SLN) on HepG2 cells.Method: 3-(4

5-Dimethylthiazole-2-yl)-2

5-diphenyltetrazolium-bromide (MTT) assay was used to detect the cytotoxicity of B-SLN on HepG2 cells

the cellular uptake experiments were observed by fluorescence microscopy

flow cytometry was used to detect the cellular uptake of cells under different conditions.Result: Both brucine solution group and B-SLN group had an inhibitory effect on the proliferation of HepG2 cells

and the inhibition rate increased with increasing of the time and the concentration of brucine

half-inhibitory concentration (IC50) of brucine group in 48

72 h were 208.5

78.5 mg·L-1

IC50 of B-SLN group in 48

72 h were 563.3

114.9 mg·L-1.When the concentration of brucine was in the range of 125-500 mg·L-1

with increasing of the drug concentration

the cellular uptake increased from 50.2% to 71.2%;when the temperature was under the condition of 4℃ and 37℃

the cellular uptake increased from 43.0% to 55.2%;within uptake time of 30-240 min

with the incubation time increasing

the cellular uptake amount increased from 9.7% to 56.4%.Conclusion: SLN administration system can significantly improve the activity of brucine against cancer cells

B-SLN has the ability to increase cellular uptake of drugs.