Evaluation of Quality Analysis Method of Lonicerae Japonicae Flos Extract

AI Guang-li; ZENG Zhen; WANG Wei; PU Xu-feng

doi:10.13422/j.cnki.syfjx.2017050022

您当前的位置：

首页 >

文章列表页 >

Evaluation of Quality Analysis Method of Lonicerae Japonicae Flos Extract

更新时间：2024-01-04

- Evaluation of Quality Analysis Method of Lonicerae Japonicae Flos Extract
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 5, Pages: 22-26(2017)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2017050022
  CLC： R284.1
- Published：2017
- 稿件说明：
移动端阅览
AI Guang-li, ZENG Zhen, WANG Wei, et al. Evaluation of Quality Analysis Method of Lonicerae Japonicae Flos Extract[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(5): 22-26.
DOI：

AI Guang-li, ZENG Zhen, WANG Wei, et al. Evaluation of Quality Analysis Method of Lonicerae Japonicae Flos Extract[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(5): 22-26. DOI： 10.13422/j.cnki.syfjx.2017050022.

摘要

目的：探索金银花提取物质量控制的专属性方法，为相关制剂的质量控制提供参考。方法：采用HPLC，流动相乙腈（A）-0.4%磷酸水溶液（B）梯度洗脱（0~15 min，5%~20% A；15~30 min，20%~30% A；30~40 min，30% A），检测波长327 nm。比较金银花提取物与山银花、杜仲叶原药材及其提取物中绿原酸含量和特征图谱，分析银黄制剂中金银花提取物的质量。结果：原药材、提取物中均是山银花所含的绿原酸含量最高，质量分数分别为3.56%~5.09%，4.62%~6.12%；金银花提取物与杜仲叶提取物无明显差异，绿原酸质量分数约4%；金银花、山银花、杜仲叶提取物酸化后绿原酸含量均提高，分别为6.90%，7.35%，6.47%。山银花、杜仲叶提取物及其酸化提取物的特征谱图与金银花提取物标准图谱相同，部分银黄制剂中检测出了杜仲叶的主要成分京尼平苷酸和山银花的主要成分灰毡毛忍冬皂苷乙。结论：金银花提取物中可能存在添加山银花和杜仲叶的情况，需加强质量控制，为金银花提取物质量标准的提高及检测方法的选择提供了重要依据。

Abstract

Objective: To explore the exclusive methods for quality control of Lonicerae Japonicae Flos extract.Method: HPLC was employed with mobile phase of acetonitrile-0.4% phosphoric acid solution for gradient elution and detection wavelength at 327 nm.The quality of Lonicerae Japonicae Flos extract in Yinhuang preparations was analyzed

the content of chlorogenic acid and specific chromatogram of Lonicerae Japonicae Flos extract were compared with the extract and original medical herb of Lonicerae Flos and Eucommiae Folium.Result: The highest contents of chlorogenic acid in the original medical herbs and its extracts were Lonicerae Flos

mass fractions were 3.56%-5.09% and 4.62%-6.12%;there was no difference between Lonicerae Japonicae Flos extract and Eucommiae Folium extract

the content of chlorogenic acid was about 4%.After acidification

the contents of chlorogenic acid in Lonicerae Japonicae Flos extract

Lonicerae Flos extract and Eucommiae Folium extract were all increased.Specific chromatogram of extract and acid extract of Lonicerae Flos and Eucommiae Folium were the same as the standard chromatogram of Lonicerae Japonicae Flos extract.Some of Yinhuang preparations had been checked geniposidic acid from Eucommiae Folium and macranthoidin B from Lonicerae Flos.Conclusion: Lonicerae Japonicae Flos extract may be added to Lonicerae Flos and Eucommiae Folium

its quality control should be strengthened.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Discussion on Technical Characteristics of National Drug Standards for Traditional Chinese Medicine Dispensing Granules

Quality Evaluation and Suggestions on Pharmacopoeia Standard of Eucommiae Folium

Analysis of Specific Chromatogram of Wenjingtang Reference Sample

Protective Effects of Chlorogenic Acid on Liver Injury in Mice

Related Author

CHEN Shengjun

LI Song

GUO Kejia

ZHANG Yuntian

ZHOU Haiqin

PU Xianglan

WANG Ping

LIANG Shaojia

Related Institution

Jiangyin Tianjiang Pharmaceutical Co. Ltd.，Jiangyin

Henan University of Chinese Medicine

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

Yanling DU Renkang Biotechnology Co. Ltd.

Institute of Traditional Chinese Medicine（TCM），Shanghai University of TCM

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰