LI Na, HU Liang, WANG Ting, et al. Variation Characteristics of Phylloplane Microbies from and Correlation with Downy Mildew[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(5): 42-46.
DOI:
LI Na, HU Liang, WANG Ting, et al. Variation Characteristics of Phylloplane Microbies from and Correlation with Downy Mildew[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(5): 42-46. DOI: 10.13422/j.cnki.syfjx.2017050042.
Variation Characteristics of Phylloplane Microbies from and Correlation with Downy Mildew
Objective: To explore the dynamic change characteristics of phylloplane microbe from Aconitum carmichaeli and its correlation with Aconitum downy mildew
with those cultivated in Jiangyou Sichuan as the research subjects.Method: The leaves with downy mildew disease at different levels were collected. Foliar microbes were extracted by combined shock shaker and ultrasound method. The count of bacteria and fungi were measured by dilution plate count method.Result: Aconitum downy mildew was associated with environmental temperature. The count of phylloplane bacteria and fungi in the leaves suffering from downy mildew of A. carmichaeli was increased as compared to healthy one
and the count of other fungi showed a more regular upward trend with the increasing degree of downy mildew disease of A. carmichaeli.Conclusion: Aconitum downy mildew from A. carmichaeli had rapid onset in its growth period at the end of March to the end of May
and the daily average temperature between 15℃ to 20℃ was more appropriate for the growth and reproduction of Aconitum downy mildew pathogens. Changes in count of phylloplane fungi from A. carmichaeli were positively correlated with the degree of Aconitum downy mildew disease.
Establishment and Optimization of DGGE Analysis Methods for Phylloplane Bacteria and Fungi of
Establishment of Molecular Detection Method for Pathogen of Aconiti Downy Mildew
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