Simultaneous Determination of Four Ingredients in Mori Cortex by HPLC

YAN Hui; GAO Huan; CUI Qing-hua; RONG Rong; LYU Qing-tao

doi:10.13422/j.cnki.syfjx.2017050053

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Simultaneous Determination of Four Ingredients in Mori Cortex by HPLC

更新时间：2024-01-04

- Simultaneous Determination of Four Ingredients in Mori Cortex by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 5, Pages: 53-56(2017)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2017050053
  CLC： O657.72;R284.1
- Published：2017
- 稿件说明：
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YAN Hui, GAO Huan, CUI Qing-hua, et al. Simultaneous Determination of Four Ingredients in Mori Cortex by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(5): 53-56.
DOI：

YAN Hui, GAO Huan, CUI Qing-hua, et al. Simultaneous Determination of Four Ingredients in Mori Cortex by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(5): 53-56. DOI： 10.13422/j.cnki.syfjx.2017050053.

摘要

目的：建立桑白皮中绿原酸、东莨菪内酯、白藜芦醇、桑色素含量测定的方法。方法：采用EclipseSB-C18色谱柱（4.6 mm×250 mm，5 μm），流动相乙腈-0.01%磷酸梯度洗脱，流速1.0 mL·min-1，柱温30℃，检测波长285 nm。结果：绿原酸、东莨菪内酯、白藜芦醇和桑色素分别在6.4~76.8 mg·L-1（r=0.999 9），5.15~61.8 mg·L-1（r=0.999 6），1.11~13.32 mg·L-1（r=0.999 6），2.76~33.12 mg·L-1（r=0.999 7）线性关系良好，平均加样回收率在98.4%~99.4%（RSD≤2%），供试品溶液在12 h内稳定，且该方法重复性良好；通过对5批桑白皮药材的测定，结果发现河南（批号140401）样品中绿原酸、东莨菪内酯、桑色素含量均较高，湖北（批号D5090202）样品中白藜芦醇含量相对最高。结论：该含量测定方法精密度高，准确度高，适合桑白皮中4种成分的含量测定。

Abstract

Objective: To develop an HPLC method for quantitative determination of chlorogenic acid

scopoletin

resveratrol and morin in Mori Cortex.Method: An Eclipse-C18 column (4.6 mm×250 mm

5 μm) was adopted with acetonitrile-0.01% phosphoric acid as the mobile phase for gradient elution. The flow rate was 1.0 mL·min-1. The detection wavelength was set at 285 nm and the column temperature was 30℃.Result: The calibration curves showed good linear relationship in the ranges of 6.4-76.8 mg·L-1 (r=0.999 9) for chlorogenic acid

5.15-61.8 mg·L-1(r=0.999 6) for scopoletin

1.11-13.32 mg·L-1 (r=0.999 6) for resveratrol and 2.76-33.12 mg·L-1(r=0.999 7) for morin. The average recoveries were between 98.4% and 99.4%(RSD≤2%). The sample solution was steady within 12 h

and this method showed good repeatability. Through the determination of 5 batches of Mori Cortex samples

results showed that the contents of chlorogenic acid

scopoletin and morin were higher in Henan samples (batch number 140401); and the content of resveratrol was higher in Hubei samples (batch number D5090202).Conclusion: The method is precise and accurate for determination of four ingredients in Mori Cortex.

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Related Institution

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