Objective: To study the mechanism of Breviscapine on cerebral ischemia reperfusion injury of rats by cytochrome (CYP) 4A1

CYP4A3

CYP4A8 pathways.Method: Totally 193 male SD rats were selected to establish the model of middle cerebral artery occlusion (MCAO). After cerebral ischemia for 2 h and reperfusion

they were injected with Breviscapine at the doses of 12

6

3 mg·kg-1

qd

for 7 days. After the final medication

tissue fluids isolated from brain tissues were prepared. Genes expressions of CYP4A1

CYP4A3 and CYP4A8 in brain tissues of rats were examined by Real-time PCR assays. The content of endothelial nitric oxide synthase (eNOS) was examined by enzyme linked immunosorbent assay(ELISA).Result: Compared with shame group

model group showed significant increase in motility (P<0.01)

volume of cerebral infarction and behavioral score (P<0.01) at 168 h

and up-regulation in the expressions of CYP4A3

CYP4A8 (P<0.01). The content of eNOS was increased in brain tissues and serum. Compared with model group

Bre 12 mg·kg-1 group and Bre 6 mg·kg-1 group showed significant reduction in motility (P<0.05)

volume of cerebral infarction (P<0.01) at 168 h

behavioral score (P<0.01) after 24 h

and down-regulation in the expressions of CYP4A3

CYP4A8 (P<0.05

P<0.01) and the content of eNOS (P<0.01) in brain tissues and serum. Bre 3 mg·kg-1 group showed significant reduction in the behavioral score (P<0.01) at 120 h and the content of eNOS (P<0.01)

and down-regulation in the expression of CYP4A3

CYP4A8 (P<0.01) in brain tissues.Conclusion: Motility

volume of cerebral infarction and behavioral score were increased by cerebral ischemia reperfusion injury

the expression of CYP4A3

CYP4A8 were up-regulated

and the content of eNOS was increased in brain tissues. Breviscapine injection could cure cerebral ischemia reperfusion injury of rats by reducing the volume of cerebral infarction

behavioral score

which may be correlated with down-regulation in the expressions of CYP4A3

CYP4A8 in brain tissues.