Effect of Ultra-filtration Extract from Angelice Sinensis Radix and Hedysari Radix on TGF- in Kidney Tissue of Rat with Diabetic Nephropathy

CHEN Zhang-min; LI Rong-ke; ZHANG Peng-fei; WAN Sheng-fang; ZHANG Ling-wei; GUO Yu-long; WANG Qiu-lan

doi:10.13422/j.cnki.syfjx.2017050113

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Effect of Ultra-filtration Extract from Angelice Sinensis Radix and Hedysari Radix on TGF- in Kidney Tissue of Rat with Diabetic Nephropathy

更新时间：2024-01-04

- Effect of Ultra-filtration Extract from Angelice Sinensis Radix and Hedysari Radix on TGF- in Kidney Tissue of Rat with Diabetic Nephropathy
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 5, Pages: 113-118(2017)
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- DOI：10.13422/j.cnki.syfjx.2017050113
  CLC： R285.5
- Published：2017
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CHEN Zhang-min, LI Rong-ke, ZHANG Peng-fei, et al. Effect of Ultra-filtration Extract from Angelice Sinensis Radix and Hedysari Radix on TGF- in Kidney Tissue of Rat with Diabetic Nephropathy[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(5): 113-118.
DOI：

CHEN Zhang-min, LI Rong-ke, ZHANG Peng-fei, et al. Effect of Ultra-filtration Extract from Angelice Sinensis Radix and Hedysari Radix on TGF- in Kidney Tissue of Rat with Diabetic Nephropathy[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(5): 113-118. DOI： 10.13422/j.cnki.syfjx.2017050113.

摘要

目的：研究当归-红芪超滤膜提取物对糖尿病肾病（DN）大鼠肾组织转化生长因子-β1（TGF-β1），Smad信号通路的调控作用，探讨其对DN大鼠的作用及产生该作用可能的机制。方法：采用一次性大剂量（60 mg·kg-1）腹腔注射链脲佐菌素（STZ）诱导建立DN大鼠模型。将DN大鼠随机分为正常组、模型组、厄贝沙坦组及当归-红芪超滤膜提取物低、中、高剂量组，之后开始灌胃给药，每天1次，8周后测空腹血糖（FBG），尿素氮（BUN），血肌酐（SCr），24 h尿蛋白；光镜观察肾组织结构变化；酶联免疫吸附测定（ELISA）检测大鼠TGF-β1，Smad2/3含量；免疫组化法检测肾组织TGF-β1，Smad2/3蛋白的表达；实时荧光定量聚合酶链式反应（Real-time PCR）法检测肾组织TGF-β1，Smad2/3的mRNA表达。结果：与正常组比较，模型组大鼠FBG，BUN，SCr，24 h尿蛋白水平明显升高，ELISA检测大鼠TGF-β1，Smad2/3含量明显升高，肾组织病理结构异常较为明显，TGF-β1，Smad2/3蛋白量表达明显升高（P<0.05，P<0.01）；与模型组比较，当归-红芪超滤膜提取物各组均不同程度降低了DN大鼠FBG，BUN，SCr，24 h尿蛋白水平（P<0.05）；ELISA检测大鼠TGF-β1，Smad2/3含量降低（P<0.05），肾组织病理结构异常得到改善，TGF-β1，Smad2/3蛋白量表达减少（P<0.05，P<0.01），TGF-β1，Smad2/3 mRNA表达减少（P<0.05，P<0.01）。结论：当归-红芪超滤膜提取物可改善DN大鼠肾功能病变，延缓DN慢性病理进展，其机制可能与其抑制TGF-β1/Smad信号通路有关。

Abstract

Objective: To study the regulatory effect of ultra-filtration extract from Angelice Sinensis Radix and Hedysari Radix(UFEASH) on transforming growth factor-β1(TGF-β1) and Smad signaling pathway of kidney tissue of rat with diabetic nephropathy (DN)

and explore its possible mechanism.Method: The rat model with DN was induced by one-time high-dose (60 mg·kg-1) intraperitoneal injection with streptozotocin (STZ). All experimental rats were divided into blank group

model group

irbesartan group and low

median

high-dose UFEASH groups

and then intragastrically administrated once a day. Fasting blood glucose

urea nitrogen (BUN)

serum creatinine (SCr)

24 h urinary protein were detected after 8 weeks. The changes in renal tissue structure were observed under light microscope. TGF-β1 and smad2/3 content of the rats were tested by ELISA. The expression of TGF-β1

Smad2/3 protein and mRNA expressions in renal tissues were detected by immunohistochemistry and Real-time PCR

respectively.Result: Compared with the normal group

the model group showed significant increases in FBG

BUN

SCr and 24 h urinary protein; ELISA showed notable increases in TGF-β1 and Smad2/3 content

remarkable renal pathological structural abnormality and notable increase in TGF-β1 and Smad2/3 protein expressions (P<0.05

P<0.01). Compared with the model group

UFEASH groups significantly reduced the level of FBG

BUN

SCr and 24 h urine protein of rats with DN (P<0.05). The levels of TGF-β1 and Smad2/3 mRNA expressions were also decreased (P<0.05). The abnormal structure of renal tissue was alleviated. The expression of TGF-β1 and Smad2/3 were reduced (P<0.05

P<0.01).Conclusion: UFEASH can improve renal function in rats with DN and delay the chronic pathological progress of DN

which may be related to the inhibition of TGF-β1/Smad signaling pathway.

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