Effect of Triterpene from Chaenomelis Fructus on Expression of Akt, NF-B and Inflammatory Factors in Adjuvant Arthritis Rats

WANG Hai-yan; QIN Hui-lin; ZHANG Yong-feng; YAN Wei-hong; HE Hai-bo; DING Hua-li; WANG Jun-zhi

doi:10.13422/j.cnki.syfjx.2017050141

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Effect of Triterpene from Chaenomelis Fructus on Expression of Akt, NF-B and Inflammatory Factors in Adjuvant Arthritis Rats

更新时间：2024-01-04

- Effect of Triterpene from Chaenomelis Fructus on Expression of Akt, NF-B and Inflammatory Factors in Adjuvant Arthritis Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 5, Pages: 141-146(2017)
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- DOI：10.13422/j.cnki.syfjx.2017050141
  CLC： R285.5
- Published：2017
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WANG Hai-yan, QIN Hui-lin, ZHANG Yong-feng, et al. Effect of Triterpene from Chaenomelis Fructus on Expression of Akt, NF-B and Inflammatory Factors in Adjuvant Arthritis Rats[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(5): 141-146.
DOI：

WANG Hai-yan, QIN Hui-lin, ZHANG Yong-feng, et al. Effect of Triterpene from Chaenomelis Fructus on Expression of Akt, NF-B and Inflammatory Factors in Adjuvant Arthritis Rats[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(5): 141-146. DOI： 10.13422/j.cnki.syfjx.2017050141.

摘要

目的：观察木瓜三萜对佐剂性关节炎（adjuvant arthritis，AA）大鼠关节滑膜组织中Akt，核转录因子-κB（NF-κB）和炎症因子表达影响，在此基础上探讨其可能机制。方法：80只雄性SD大鼠随机分为正常组、模型组、木瓜总三萜中、高剂量组（50，100 mg·kg-1），除正常组外，其余大鼠右后足跖注射弗氏佐剂造模，木瓜三萜（50，100 mg·kg-1）灌胃（ig）给药，足容积法测量致炎侧足肿胀、进行关节评分；光学显微镜观察关节滑膜病理变化；酶联免疫吸附测定（ELISA）法检测大鼠关节滑膜组织中肿瘤坏死因子-α（TNF-α），白细胞介素-1β（IL-1β），白细胞介素-6（IL-6）和白细胞介素-8（IL-8）含量，蛋白质免疫印迹法（Western blot）检测关节滑膜组织中蛋白激酶B（Akt），磷酸化蛋白激酶B（p-Akt）和人核转录因子-κB p65（NF-κB p65）表达水平。结果：与正常组比较，大鼠致炎后14 d关节肿胀度达峰值，可见模型组关节滑膜组织炎症反应，如滑膜组织增生，炎细胞浸润；关节组织中TNF-α，IL-1β，IL-6和IL-8含量明显上升，p-Akt和NF-κB p65表达增多（P<0.01）；与模型组比较，木瓜三萜中、高剂量组能显著抑制AA大鼠足爪肿胀、降低多发性关节评分及改善关节滑膜组织病理变化，降低滑膜组织病理评分，降低关节滑膜组织中TNF-α，IL-1β，IL-6和IL-8含量及p-Akt和NF-κB p65蛋白表达水平（P<0.05，P<0.01）。结论：木瓜三萜具有显著的抗炎作用，抑制Akt/NF-κB信号通路激活及促炎因子生成可能是其发挥抗炎作用的重要机制之一。

Abstract

Objective: To investigate the effect of triterpene from Chaenomelis Fructus on the expression levels of Akt

NF-κB and inflammatory factors in synovial tissues of joint in adjuvant arthritis (AA) rats

and explore its possible mechanism.Method: Totally 80 male SD rats were randomly divided into normal group

model group

triterpene from Chaenomelis Fructus high and middle dose groups (50

100 mg·kg-1). The models were established by injection of adjuvant on right hind paws of the rats. Triterpene from Chaenomelis Fructus (50

100 mg·kg-1) was given by ig administration

and foot volume method was used to determine paw swelling and joint scores. The pathological changes of synovium were observed under optical microscope; and the levels of tumor necrosis factor-α (TNF-α)

interleukin 1β (IL-1β)

interleukin 6 (IL-6) and interleukin 8(IL-8) in joint synovial tissues were measured by using ELISA. The protein expression levels of protein kinase B (Akt)

phospho-Akt (p-Akt) and nuclear transcription factor-κB p65 (NF-κB p65) in joint synovial tissues were measured by western blotting.Result: After 14days of joint inflammation

paw swelling reached the peak as compared with the normal group. The joint synovial hyperplasia and cell infiltration were seen obviously in model rats; the levels of TNF-α

IL-1β

IL-6 and IL-8 in joint synovial tissues were significantly increased; the NF-κBp65 and p-Akt protein expression levels were increased (P<0.01). As compared with the model group

triterpene from Chaenomelis Fructus (50 and 100 mg·kg-1) significantly decreased arthritis score

alleviated paw swelling

decreased the levels of TNF-α

IL-1β

IL-6 and IL-8 in joint synovial tissues; and significantly down-regulated the protein expression levels of p-Akt and NF-κB p65 (P<0.05

P<0.01).Conclusion: Triterpene from Chaenomelis Fructus has a significant anti-inflammatory effect. The mechanism maybe related to inhibiting Akt/NF-κB signaling pathway and activating the pro-inflammatory cytokines production.

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