Objective: To predict the active compounds

therapy targets of Liuwei Dihuangtang (LWDHT) as well as possible diseases

and explore the molecular mechanism of its compatibility and its efficacy on diseases under the guidance of ‘treating different diseases with the same method’.Method: Oral bioavailability (OB) and drug-likeness (DL) based on TCMSP database were used to screen the active compounds of LWDHT and its related diseases. Therapy targets were obtained from the Drugbank database

and then the Chinese herbal medicine (CHM) compound-targets-disease network was constructed by using cytoscape software. The network topology analysis

target function and signaling pathway analysis were also performed.Result: The 42 of 507 compounds in LWDHT were screened out (OB≥30%

drug-likeness index≥0.18).The 221 related targets were found and 9 categories of diseases involving 56 diseases were enriched

among which tumor

cardiovascular and respiratory diseases were most closely correlated with the formula. The compatibility of LWDHT

‘Sanbu’ with reinforcing effect and ‘Sanbu’ with reducing effect can regulate cellular apoptosis

metabolism

proliferation

and vascular smooth muscle contraction. Besides

LWDHT also can regulate protein synthesis modification and secretion. The ‘Sanbu’ with reinforcing effect can regulate transmembrane transport activity of ion

blood pressure

the metabolic process of prostaglandin and retinoic acid. The ‘Sanbu’ with reducing effect can regulate cell signaling and biosynthetic process.Conclusion: In this study

42 compounds were screened out as the potential effective compounds of LWDHT

and 221 targets and 56 diseases were predicted to be associated with the formula. The compatibility of ‘Sanbu’ and ‘Sanbu’ made 1+1>2 synergistic effect mechanism. LWDHT owned multiple targets and multiple pathways to treat various diseases under the guidance of ‘treating different diseases with the same method’.