ZHAO Qi-miao, SHAN Guo-shun, CHEN Jiang-ning, et al. Preliminary Study on Quality Evaluation of Arisaema Cum Bile[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(6): 28-31.
DOI:
ZHAO Qi-miao, SHAN Guo-shun, CHEN Jiang-ning, et al. Preliminary Study on Quality Evaluation of Arisaema Cum Bile[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(6): 28-31. DOI: 10.13422/j.cnki.syfjx.2017060028.
Preliminary Study on Quality Evaluation of Arisaema Cum Bile
Objective: To establish a determination method of total cholic acid
hyodeoxycholic acid and chenodeoxycholic acid in Arisaema Cum Bile
which could provide an valuable mean to control its quality. Method: The analysis was performed on ultraviolet spectrophotometry to determine the content of total cholic acid in Arisaema Cum Bile with chenodeoxycholic acid as marker substance.HPLC-CAD was used to analyze determination of hyodeoxycholic acid and chenodeoxycholic acid in Arisaema Cum Bile. Result: In the range of 0-2.435 g·L-1
it showed good linear relationships between the concentration of chenodeoxycholic acid and absorbance.The average recovery was 98.98% with RSD of 1.0%.Hyodeoxycholic acid and chenodeoxycholic acid were linear with the peak area in the range of 4.7-94
5.44-108.8 mg·L-1
the average recovery was 99.53% and 99.58%
respectively. Conclusion: This method is simple
accurate and suitable for the quality evaluation of Arisaema Cum Bile.
Current Situation and Discussion of Quality Standards of Arisaema Cum Bile
Determination of Contents of Hyodeoxycholic Acid and Chenodeoxycholic Acid in Hydrolysate of Fel Suillus by HPLC
Age Discrimination Based on Volatile Components of Arisaema Cum Bile
Isolation and Identification of Arisaema Cum Bile Fermentation Strain and Preliminary Investigation on Its Compound Strain Fermentation
Analysis of Q-markers of Arisaema Cum Bile Acting on Stroke Based on UPLC-QTOF-MS/MS and TCMIP v2.0
Related Author
LI Xin-yi
XIE Da-shuai
XU Min
LIU Yu-jie
LI Ming-jian
AI Li
WU Chun-jie
CHEN Yue
Related Institution
Beijing Shuangqiao Yanjing Chinese Medicine Pieces Factory
Institute of TCM Health Industry,China Academy of Chinese Medical Sciences
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School of Traditional Chinese Medicine(TCM), Guangdong Pharmaceutical University
School of Pharmacy,Liaoning University of Traditional Chinese Medicine