WANG Jun-xiu, ZHANG Xue-lan, WANG Qiu-hong, et al. Comparison on HPLC Fingerprints of Different Processed Products of Armeniacae Semen Amarum[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(6): 32-34.
DOI:
WANG Jun-xiu, ZHANG Xue-lan, WANG Qiu-hong, et al. Comparison on HPLC Fingerprints of Different Processed Products of Armeniacae Semen Amarum[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(6): 32-34. DOI: 10.13422/j.cnki.syfjx.2017060032.
Comparison on HPLC Fingerprints of Different Processed Products of Armeniacae Semen Amarum
Objective: To set up the HPLC fingerprint chromatograms analytical methods of different processed products of Armeniacae Semen Amarum with the personality characteristics. Method: 50% Methanol was used for ultrasonic extraction of three processed products of Armeniacae Semen Amarum.HPLC was employed with mobile phase of acetonitrile-0.1% phosphoric acid water solution for gradient elution
the column temperature at 25℃
the detection wavelength at 225 nm
the flow rate of 1.0 mL·min-1.HPLC fingerprint differences of different processed products of Armeniacae Semen Amarum were compared. Result: There were significant differences in fingerprints between crude products and processed products
but fingerprints of blazed processed products and stir-fried products were relatively similar. Conclusion: This estabilished method can be used for determining fingerprint chromatograms of different processed products of Armeniacae Semen Amarum.HPLC fingerprints have changed significantly after being processed.