Objective: To study the correlation between the GC-MS fingerprints of volatile oil from Angelicae Sinensis Radix of different regions and its effect on proliferation. Method: The volatile oil from Angelicae Sinensis Radix was extracted by steam distillation

and GC-MS analysis technology was used to establish the fingerprints of volatile oil from 44 batches of Angelicae Sinensis Radix of different regions. Mass spectrometry database and retention index were used to identify the chemical components indicated in various chromatographic peaks

and then Methyl thiazolyltetazolium(MTT) was used to investigate the effect of volatile oil from Angelicae Sinensis Radix of different regions on HUVECs proliferation. Finally

the fingerprint-effect relationship of volatile oil and promoting HUVECs proliferation effect could be established and analyzed by linear regression analysis. Result: Linear regression analysis showed that

X1 and X2 peaks were negatively correlated with cell proliferation rate

and other peaks showed positive correlation. Among them

X3

X6 and X7 peaks had larger linear regression coefficient

showing main constituents for promoting HUVECs proliferation. Conclusion: This method can quickly and effectively establish the spectrum-effect relationship for volatile oil from Angelicae Sinensis Radix

providing experimental basis for studying their pharmacological properties.