Targeted Profiling qHNMR for Determination of Gentiopicroside and Loganic Acid in 4 Species of Gentianae Macrophyllae Radix

YANG Xiao-rui; ZHANG Kai; LI Ze-yun; WANG Zheng-tao

doi:10.13422/j.cnki.syfjx.2017060098

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Targeted Profiling qHNMR for Determination of Gentiopicroside and Loganic Acid in 4 Species of Gentianae Macrophyllae Radix

更新时间：2024-01-04

- Targeted Profiling qHNMR for Determination of Gentiopicroside and Loganic Acid in 4 Species of Gentianae Macrophyllae Radix
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 6, Pages: 98-103(2017)
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- DOI：10.13422/j.cnki.syfjx.2017060098
  CLC： R284.1
- Published：2017
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YANG Xiao-rui, ZHANG Kai, LI Ze-yun, et al. Targeted Profiling qHNMR for Determination of Gentiopicroside and Loganic Acid in 4 Species of Gentianae Macrophyllae Radix[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(6): 98-103.
DOI：

YANG Xiao-rui, ZHANG Kai, LI Ze-yun, et al. Targeted Profiling qHNMR for Determination of Gentiopicroside and Loganic Acid in 4 Species of Gentianae Macrophyllae Radix[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(6): 98-103. DOI： 10.13422/j.cnki.syfjx.2017060098.

摘要

目的：建立靶向轮廓分析核磁定量法（TP-qHNMR）同时测定秦艽、麻花艽、粗茎秦艽、小秦艽4种秦艽中龙胆苦苷、马钱苷酸的含量。方法：采集龙胆苦苷、马钱苷酸标准核磁共振氢谱，采用Chenomx NMR suit自建龙胆苦苷与马钱苷酸核磁共振氢谱数据库；秦艽药材采用甲醇超声法提取，提取液吹干以氘代甲醇复溶，同等条件下于记录核磁共振氢谱；基于Chenomx对谱图进行预处理与靶向轮廓分析定量；并采用HPLC-UV法验证TP-qHNMR法测定结果。结果：基于Chenomx的TP-qHNMR法测定秦艽中龙胆苦苷与马钱苷酸含量测定结果准确可靠，与HPLC-UV法无显著差异。所考察的4种秦艽中龙胆苦苷与马钱苷酸含量高低依次为：粗茎秦艽 > 秦艽 > 麻花艽 > 小秦艽。结论：所建立的TP-qHNMR法准确、可靠，可用于秦艽的质量控制，促进了核磁定量技术在中药质量控制中的应用。

Abstract

Objective: To establish a targeted profiling qHNMR (TP-qHNMR) method for content determination of gentiopicroside and loganic acid in 4 kinds of Gentianae Macrophyllae Radix (Gentiana macrophμlla

G. straminea

G. crassicaulis and G. dahurica). Method: TP-qHNMR software of Chenomx NMR suit was employed and standard 1H-NMR spectra of gentiopicroside and loganic acid were collected. Gentianae Macrophyllae Radix was extracted by using methanol ultrasonic method and the dried extraction was reconstituted in CH3OD before spectra were recorded. All spectra were recorded in the same condition

and then targeted profiling quantification was achieved in reference to chemical shape indicator (CSI) by Chenomx profiler and further validated by HPLC-UV method. Result: The established TP-qHNMR method was accurate and reliable for determination of gentiopicroside and loganic acid in Gentianae Macrophyllae Radix

showing no significant difference with HPLC-UV method. The contents of gentiopicroside and loganic acid in 4 kinds of Gentianae Macrophyllae Radix were as follows:G. crassicaulis > G. macrophμlla > G. straminea > G. dahurica. Conclusion: TP-qHNMR was accurate and reproducibly for quality control of Gentianae Macrophyllae Radix

and can be widely applied for quality control of traditional Chinese medicine.

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