ZHANG Nan-qi, LI Ping-ya, WANG Cui-zhu, et al. Absorbed Components Analysis of Total Saponins from Panacis Quinquefolii Radix Fruit in Rat Serum by UPLC-Q-TOF/MS[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(6): 117-124.
DOI:
ZHANG Nan-qi, LI Ping-ya, WANG Cui-zhu, et al. Absorbed Components Analysis of Total Saponins from Panacis Quinquefolii Radix Fruit in Rat Serum by UPLC-Q-TOF/MS[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(6): 117-124. DOI: 10.13422/j.cnki.syfjx.2017060117.
Absorbed Components Analysis of Total Saponins from Panacis Quinquefolii Radix Fruit in Rat Serum by UPLC-Q-TOF/MS
Objective: To analyze and identify the constituents in rat serum after oral administration of total saponins from Panacis Quinquefolii Radix fruit (TSPQF)
and provide a reference for the pharmacodynamics material basis research. Method: The male Wistar rats were selected as experimental animals
rats were intragastric administered TSPQF at a dose of 2.6 g·kg-1.The serum samples were collected and pretreated.The absorbed prototype constituents and their metabolites in rat plasma were rapidly analyzed and identified by combination of UPLC-Q-TOF/MS and multivariate statistical analysis. Result: A total of 9 absorbed components were detected and identified
including 5 prototype components
such as pseudoginsenoside F11
ginsenoside Rc
Rb3
Rd and protopanaxatriol.The other 4 metabolites were protopanaxadiol
pseudoginsenoside RT5
ginsenoside CK
Rh2
respectively. Conclusion: These nine constituents absorbed into blood may be the potential bioactive components in TSPQF
this result can provide a scientific basis for clarifying its substance basis of pharmacodynamics.
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