Oxymatrine Protects Against Liver Injury Induced by ConA in Mice Through Suppressing Host Immune Response

SANG Xiu-xiu; WANG Rui-lin; HAN Yan-zhong; CUI He-rong; YIN Ping; HE Lan-zhi; LIU Hui-min; LIU Shi-jing; XIAO Xiao-he; BAI Zhao-fang

doi:10.13422/j.cnki.syfjx.2017060125

您当前的位置：

首页 >

文章列表页 >

Oxymatrine Protects Against Liver Injury Induced by ConA in Mice Through Suppressing Host Immune Response

更新时间：2024-01-04

- Oxymatrine Protects Against Liver Injury Induced by ConA in Mice Through Suppressing Host Immune Response
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 6, Pages: 125-130(2017)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2017060125
  CLC： R285.5
- Published：2017
- 稿件说明：
移动端阅览
SANG Xiu-xiu, WANG Rui-lin, HAN Yan-zhong, et al. Oxymatrine Protects Against Liver Injury Induced by ConA in Mice Through Suppressing Host Immune Response[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(6): 125-130.
DOI：

SANG Xiu-xiu, WANG Rui-lin, HAN Yan-zhong, et al. Oxymatrine Protects Against Liver Injury Induced by ConA in Mice Through Suppressing Host Immune Response[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(6): 125-130. DOI： 10.13422/j.cnki.syfjx.2017060125.

摘要

目的：本文旨在研究氧化苦参碱（OMT）对刀豆蛋白A（ConA）所致小鼠免疫性肝损伤的保护作用。方法：Balb/C小鼠随机分为5组，分别为正常组，模型组，氧化苦参碱低、高剂量（60，120 mg·kg-1）组和双环醇（156 mg·kg-1）阳性药组。连续给药5 d，末次给药30 min后，除正常组外，其余各组均尾静脉注射ConA（15 mg·kg-1）建立肝损伤模型。造模8 h后，检测血清中丙氨酸氨基转移酶（ALT），天门冬氨酸氨基转移酶（AST），总胆红素（TBIL）的表达。采用Luminex多因子检测的方法，检测血清干扰素-γ（IFN-γ），肿瘤坏死因子-α（TNF-α），白细胞介素-4（IL-4），IL-6，IL-10，IL-27表达，苏木精-伊红（HE）染色观察肝组织病理改变。流式细胞术检测小鼠脾淋巴细胞CD4+IL-17A的表达，CD4+CD25+FoxP3+调节性T细胞（Treg）亚群的比例。结果：与正常组比较，ConA模型组ALT，AST，TBIL表达明显增高（P<0.05，P<0.01）。与模型组比较，双环醇和OMT低高剂量组均可明显降低ALT活性（P<0.05，P<0.01）。OMT高剂量可明显抑制AST表达（P<0.05），OMT低高剂量均可抑制TBIL表达（P<0.05），其余各组无统计学差异。各给药组均能不同程度缓解肝脏病理改变，减少炎性细胞浸润。OMT高剂量组血清IL-10，IL-27水平与模型组比较显着升高（P<0.05）。流式细胞术检测结果显示，OMT高剂量组可明显降低CD4+IL-17A表达（P<0.05），上调CD4+CD25+FoxP3+Treg比例（P<0.05）。结论：OMT对ConA诱导小鼠免疫性肝损伤具有较好的保护作用，抑制CD4+IL-17A的表达，上调CD4+CD25+FoxP3+Treg比例可能是其主要的作用机制。

Abstract

Objective: To investigate the immunomodulatory effect of oxymatrine on concanavalin A(ConA)-induced liver injury in mice. Method: In the present study

Balb/C mice were randomly divided into five groups:normal group

model group

low and high-dose oxymatrine groups (60

120 mg·kg-1) and bicyclol group (156 mg·kg-1). The mice in the treated groups were administered with oxymatrine (intraperitoneally) or bicyclol (orally) for 5 consecutive days. 30 min after the last medication

all the mice except the normal group were injected with 15 mg·kg-1 of ConA intravenously to establish liver injury models. 8 hours later

blood samples were taken to test the levels of alanine transaminase(ALT)

aspartate aminotransferase(AST)

and total bilrubin(TBIL) in serum

and the liver tissues were taken for HE-staining to evaluate the histopathology changes. The expression levels of serum cytokines interferon-γ(IFN-γ)

tumour necrosis factor-α(TNF-α)

interleukin-4(IL-4)

interleukin-6(IL-6)

interleukin-10(IL-10) and interleukin-27(IL-27) were measured by luminex. Spleen lymphocytes were isolated and the expression levels of CD4+IL-17A and the percentage of CD4+CD25+FoxP3+Treg were tested by flow cytomerty. Results showed that

after con-A injection

the expression levels of ALT

AST and TBIL were increased (P<0.05

P<0.01) as compared with the normal group. As compared with the model group

bicyclol and oxymatrine (60

120 mg·kg-1) could markedly decrease the activity of ALT (P<0.05

P<0.01); oxymatrine at 120 mg·kg-1 could significantly decrease the levels of AST (P<0.05)

and oxymatrine at both 60

120 mg·kg-1 could decrease the levels of TBIL (P<0.05). There were no statistical differences in other groups. All treatment groups could relieve the liver pathological changes to different degrees

and reduce the infiltration of inflammatory cells. After treatment with high dose OMT

the levels of IL-10 and IL-27 were significantly increased (P<0.05). Flow cytomerty results revealed that high dose OMT could significantly decrease the expression levels of CD4+IL-17A (P<0.05) and up-regulate the percentage of CD4+CD25+foxP3+Treg cells (P<0.05). Conclusion: oxymatrine could alleviate ConA-induced liver injury by inhibiting the expression of IL-17A and promoting the proliferation of Treg cells.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Analysis of Mechanism of Antidepressant Effect of Sophora flavescens Seed Extract

Effect of Dendrobii Caulis Mixture on Cell Inflammatory Response and Apoptosis in Diabetic Rats with Nonalcoholic Fatty Liver Disease Based on NF-κB/NLRP3/IL-1β Signaling Pathway

Protective Effect of Ethanolic Extract of Hemsleya chinensis on HCl/Ethanol-induced Acute Gastric Ulcer in Rats Based on p38 MAPK/NF-κB Signaling Pathway

Anti-breast Cancer Activities in Vitro of Oxymatrine Combined with Bevacizumab： An Exploration Focusing on EMT Regulation Through Wnt/β-catenin

Biological Evaluation for Quality Control of Water Extract of Qingjin Huatantang Based on Phagocytic and Secretory Functions of Macrophages

Related Author

ZHU Tian

WANG Ru

BIAN Lihua

LI Wenjing

LI Jie

CHEN Liangmian

WANG Zhimin

GAO Huimin

Related Institution

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

Beijing Health Vocational College

University of Chinese Academy of Sciences

Institute of Psychology，Chinese Academy of Sciences

The Third People's Hospital Affiliated to Fujian University of TCM

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰