Secondary Metabolites of An Endophytic Fungus  sp. Isolated from

TONG Yong-chun; LIU Ya-ru; HE Lei; FAN Jia-jia; JIANG Chun-li; SUN Yi

doi:10.13422/j.cnki.syfjx.2017070064

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Secondary Metabolites of An Endophytic Fungus sp. Isolated from

更新时间：2024-01-04

- Secondary Metabolites of An Endophytic Fungus sp. Isolated from
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 7, Pages: 64-67(2017)
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- DOI：10.13422/j.cnki.syfjx.2017070064
  CLC： R284.1
- Published：2017
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TONG Yong-chun, LIU Ya-ru, HE Lei, et al. Secondary Metabolites of An Endophytic Fungus sp. Isolated from [J]. Chinese journal of experimental traditional medical formulae, 2017, 23(7): 64-67.
DOI：

TONG Yong-chun, LIU Ya-ru, HE Lei, et al. Secondary Metabolites of An Endophytic Fungus sp. Isolated from [J]. Chinese journal of experimental traditional medical formulae, 2017, 23(7): 64-67. DOI： 10.13422/j.cnki.syfjx.2017070064.

摘要

目的：研究藏族药水母雪莲内生真菌Alterraria sp.的次级代谢产物。方法：从植物水母雪莲中分离得到内生菌Alterraria sp.，在考察培养条件后，对该菌种进行了扩大培养。运用反复硅胶柱色谱，LH-20凝胶柱色谱、开放ODS柱色谱、高效液相色谱等方法，并结合液质联用分析技术，对菌液的总萃取物进行分离纯化；并根据化合物的理化常数测定及光谱学（1D和2D NMR，MS等）数据分析鉴定所得化合物结构。结果：对水母雪莲中分离得到的内生真菌交链孢菌属Alterraria sp.进行次级代谢产物的研究，共得到9个化合物，分别鉴定为spirotryprostatin A（1），对羟基苯甲醛（2），4-羟基苯基-2’-羟基丙酯（3），二氢对甲氧基肉桂酸（4），4-甲氧基苯甲酸（5），对羟基苯乙酸甲酯（6），4-羟基苯乙醇（7），对羟基苯甲酸（8），3-苯基丙酸（9）。细胞毒测试结果表明，从TPXa中分离得到的化合物1对肺癌细胞株A549具有较强的增殖抑制活性，其IC50为2.0 mg·L-1。结论：以上化合物均为首次从交链孢菌属真菌中分离得到。

Abstract

Objective: To study the secondary metabolites of an endophytic fungus Alterraria sp. isolated from Saussurea medusa. Method: Alterraria sp. was isolated from the medical plant S. medusa.The strain was cultured in a large-scale after the culture conditions had been optimized. The secondary metabolites were isolated by chromatography methods such as silica gel

Sephadex LH-20 columns

ODS flash column and HPLC

and the analysis of LC-MS. The structures of these compounds were identified by theirphysico-chemical constants and the NMR and MS data. Result: Nine compounds were isolated and identified as spirotryprostatin A (1)

4-hydroxybenzoic aldehyde(2)

4-hydroxyphenyl-2'-hydroxypropanoate (3)

dihydro-p-methoxycinnamic acid(4)

4-methoxybenzoic acid(5)

p-hydroxymethylphenylacetate(6)

4-hydroxyphenyl ethanol(7)

4-hydroxybenzoic acid (8)

3-phenylpropanoic acid(9). All compounds were tested for their inhibitory activities against human lung adenocarcinoma strain A-549.Compound 1 exhibited strong cytotoxic activity against A549 with IC50 value of 2.0 mg·L-1. Conclusion: All the compounds were isolated from Alterraria for the first time.

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