Gliotoxins Isolated from Endophyic  sp. of Astragali Radix and Their Antimicrobial Activity

ZHANG Hong-chi; LIU Rui; LI Hui; AN Zhi-peng; ZHOU Feng

doi:10.13422/j.cnki.syfjx.2017070068

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Gliotoxins Isolated from Endophyic sp. of Astragali Radix and Their Antimicrobial Activity

更新时间：2024-01-04

- Gliotoxins Isolated from Endophyic sp. of Astragali Radix and Their Antimicrobial Activity
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 7, Pages: 68-72(2017)
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- DOI：10.13422/j.cnki.syfjx.2017070068
  CLC： R284
- Published：2017
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ZHANG Hong-chi, LIU Rui, LI Hui, et al. Gliotoxins Isolated from Endophyic sp. of Astragali Radix and Their Antimicrobial Activity[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(7): 68-72.
DOI：

ZHANG Hong-chi, LIU Rui, LI Hui, et al. Gliotoxins Isolated from Endophyic sp. of Astragali Radix and Their Antimicrobial Activity[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(7): 68-72. DOI： 10.13422/j.cnki.syfjx.2017070068.

摘要

目的：研究恒山黄芪内生真菌Penicillium sp.的gliotoxin类代谢产物和其对黄芪白粉病原菌Erysiphe pisi.的抑制作用。方法：次生代谢产物的分离纯化采用反复硅胶柱色谱法，Sephadex LH-20凝胶色谱法和HPLC方法，并通过化学方法结合核磁共振分析和质谱鉴定其化学结构，抑菌活性测试方法采用最小抑制浓度法。结果：从恒山黄芪内生真菌Penicillium sp.的固体发酵萃取物中分离得到7个gliotoxin类代谢产物，经波谱分析分别鉴定为gliotoxin（1），bisdethiobis（methylthio）gliotoxin（2），dehydroxybisdethiobis（methylthio）gliotoxin（3），dehydrogliotoxin（4），6-deoxy-5a，6-didehydrogliotoxin（5），didehydrobisdethiobis（methylthio）gliotoxin（6），bis-N-norgliovietin（7），抑菌活性测试结果显示，化合物1，4和5对黄芪白粉病原菌显示出强抑制作用，最小抑菌浓度（MIC）分别达到1.56，0.78，6.25 mg·L-1。结论：所有化合物都是首次从黄芪内生真菌发酵产物中分离得到，黄芪内生真菌Penicillium sp.可以作为潜在的产生gliotoxin类代谢产物的菌种资源。

Abstract

Objective: To study on gliotoxin metabolites from endophytic Penicillium sp. of Astragali Radix and investigate their antimicrobial activity to Erysiphe pisi. Method: The compounds were isolated and purified by repeated silica gel column chromatography

Sephadex LH-20 gel chromatography

and HPLC method; their chemical structures were identified by chemical methods combined with nuclear magnetic resonance analysis and mass spectrometry. The MIC values for E. pisi. were determined as sensitivity to the compounds in biological evaluation. Result: Seven gliotoxins were obtained and elucidated as gliotoxin (1)

bisdethiobis(methylthio) gliotoxin (2)

dehydroxybisdethiobis(methylthio) gliotoxin (3)

dehydrogliotoxin (4)

6-deoxy-5a

6-didehydrogliotoxin (5)

didehydrobisdethiobis (methylthio) gliotoxin (6)

and bis-N-norgliovietin (7). Bacteriostasic activity test results showed that compounds 1

4 and 5 had strong inhibitory activity for E. pisi

MIC values of 1.56

0.78 and 6.25 mg·L-1. Conclusion: All compounds were isolated from the endophytic fungi of Astragali Radix for the first time

and the endophytic fungus Aspergillus sp. from the stems of Astragali Radix

is a promising source for gliotoxins.

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