WEN Bin-yu, YAN Yan, WANG Wei-hao, et al. Pharmacokinetics of Effective Components After Oral Administration of Poria Triterpenoids Extract with Different Forms in Rats[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(7): 97-101.
DOI:
WEN Bin-yu, YAN Yan, WANG Wei-hao, et al. Pharmacokinetics of Effective Components After Oral Administration of Poria Triterpenoids Extract with Different Forms in Rats[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(7): 97-101. DOI: 10.13422/j.cnki.syfjx.2017070097.
Pharmacokinetics of Effective Components After Oral Administration of Poria Triterpenoids Extract with Different Forms in Rats
Objective: To establish a method of UPLC-Q-TOF/MS for determination of tumulosic acid and dehydro-tumulosic acid in rat plasma after oral administration of Poria triterpenoids extract powder and soft capsules content
differences of pharmacokinetics of tumulosic acid and dehydro-tumulosic acid in two forms were compared. Method: Liquid-liquid extraction was adopted to extract plasma samples with ethyl acetate
taking glycyrrhetic acid as internal standard
good chromatographic separation was achieved with the mobile phase consisting of 0.1% acetic acid in acetonitrile and 0.1% acetic acid in water for gradient elution.Mass conditions were as following:positive sensitive mode
capillary voltage of 3 kV
ion source temperature of 120℃
desolvent temperature of 500℃ and desolvation gas flow of 800 L·h-1. Result: Cmax and AUC0-t of tumulosic acid and dehydro-tumulosic acid in soft capsules content were greater than those of powder.Tmax of tumulosic acid in soft capsules content was longer than that of the powder
but Tmax of dehydro-tumulosic acid was lower than that of powder. Conclusion: These results indicate that vegetable oil can promote the absorption of tumulosic acid and dehydro-tumulosic acid.It is probably that the different form of Poria triterpenoids extract lead to the change of absorption rate due to the different chemical structure of tumulosic acid and dehydro-tumulosic acid.
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