Objective: To investigate the effect of Miao medicine Jinwu Jiangu decoction on the expression levels of nuclear transcription factor-κB (NF-κB) p65

IκB kinase-α (IKK-α) and IκB kinase-β (IKK-β)in the fibroblast-like synoviocyte of rheumatoid arthritis (FLS of RA) and explore its mechanism. Method: FLS of RA models were established in vitro through culturing

and the second to third generation cells were randomly divided into 6 groups:blank group

tripterygium wilfordii group

prednisolone group

Jinwu Jiangu decoction high

middle and low dose treatment groups(28.8

9.6

3.2 g·kg-1). After treatment for 24 h

the morphological changes were observed in all groups. Then flow cytometry was adopted to detect the apoptosis rate of the processed fibroblast-like synoviocyte of RA and Western blot was used to detect the expression levels of NF-κB p65

IKK-α and IKK-β of the fibroblast-like synoviocyte of rheumatoid arthritis. Result: As compared with the control group

the apoptosis rate of the fibroblast-like synoviocyte of rheumatoid arthritis was gradually increased with the higher concentration of Jinwu Jiangu decoction (P<0.05)

and the expression levels of NF-κB p65

IKK-α and IKK-β were significantly decreased (P<0.05). Conclusion: Miao medicine Jinwu Jiangu decoction can induce the apoptosis of fibroblast-like synoviocyte of rheumatoid arthritis

and reduce the expression levels of NF-κB p65

IKK-α and IKK-β in fibroblast-like synoviocyte of rheumatoid arthritis.