Objective: To optimize formulation and preparation technology of chlorin e6 (Ce6) magnetic sound sensitive nano-liposomes

and establish its quality evaluation method. Method: The liposomes was prepared by thin film dispersion-vacuum freeze drying technique

taking encapsulation efficiency as index

orthogonal test was adopted to investigate effects of types of organic phase

hydration medium

temperature and time on preparation technology and effects of ratio of lecithin-cholesterol

ratio of lecithin-Ce6

ratio of Ce6-magnetic particle and dosage of tween-80 on prescription.Morphology was examined by transmission electron microscope

particle size was determined by laser particle sizer

in vitro release behavior of sound sensitivity was studied by dynamic dialysis and compared with ordinary liposomes. Result: Optimum preparation technology was as following:chloroform as the organic phase

water for hydration medium

hydration temperature at 55℃ for 50 min;optimum formula was as following:lecithin-cholesterol (5:1)

lecithin-Ce6 (1:50)

Ce6-magnetic particle (1:2) and tween-80 amount of 0.5%.Morphology of the liposomes showed sphere structure and rounded appearance with uniform diameter.Average encapsulation efficiency was 90.28%

rate of leakage was 3.28% and particle size was 176.9 nm.With increasing of ultrasonic time (0-150 s) and intensity (0-2.0 W·cm-2)

the in vitro cumulative release of the nano-liposomes increased. Conclusion: Ce6 magnetic sound sensitive nano-liposomes has high encapsulation efficiency and good sound sensitive characteristics in vitro.