Objective: To evaluate the quality of Bupleuri Radix produced in Ningxia by HPLC finger-print and near-infrared spectrum for 13 batches of Bupleuri Radix and its control medicinal herb. Method: HPLC finger-print and near-infrared spectroscopy were built up for comparing the similarities between the 13 batches of Bupleuri Radix and its control medicinal herb

respectively. Furthermore

the paired t-test was carried out to analyze common peak areas and near-infrared spectrum between the 13 batches of Bupleuri Radix and control medicinal herb

respectively. Result: The results showed that the similarities of HPLC finger-print and near-infrared spectrum for the 13 batches of Bupleuri Radix and control medicinal herb were more than 0.901 and 0.982

respectively. Moreover

paired sample t-test showed no significant difference between common peak areas of the 13 batches of Bupleuri Radix produced in Ningxia and control medicinal herb. This verified a high consistency between the 13 batches of Bupleuri Radix and control medicinal herb. Conclusion: On the basis of methods of chromatographic finger-print and near-infrared spectrum

this study applied paired sample t-test to verify analysis results

which suggested no significant difference between the 13 batches of Bupleuri Radix produced on Ningxia and control medicinal herb. The study used two comprehensive detection means

which could avoid erroneous judgment caused by uncertain threshold values of similar degree in the chromatographic finger-print and provide a reliable basis for the analysis results and new ideas for improving the quality control method of traditional Chinese medicinal herbs.