Isolation and Identification of Phenolic Constituents of Sanguisorbae Radix

WANG Ze-yu; WANG Li-na; QIU Ling; LI Sha; FENG Dan; GUO Da-le; DENG Yun

doi:10.13422/j.cnki.syfjx.2017080082

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Isolation and Identification of Phenolic Constituents of Sanguisorbae Radix

更新时间：2024-01-04

- Isolation and Identification of Phenolic Constituents of Sanguisorbae Radix
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 8, Pages: 82-85(2017)
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- DOI：10.13422/j.cnki.syfjx.2017080082
  CLC： R284
- Published：2017
- 稿件说明：
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WANG Ze-yu, WANG Li-na, QIU Ling, et al. Isolation and Identification of Phenolic Constituents of Sanguisorbae Radix[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(8): 82-85.
DOI：

WANG Ze-yu, WANG Li-na, QIU Ling, et al. Isolation and Identification of Phenolic Constituents of Sanguisorbae Radix[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(8): 82-85. DOI： 10.13422/j.cnki.syfjx.2017080082.

摘要

目的：对蔷薇科地榆属植物中地榆的酚酸类化学成分进行分离并对分离得到的单体化合物进行结构鉴定。方法：将地榆干燥切片10 kg粉碎，加70%乙醇室温渗漉提取，收集渗滤液，将渗滤液减压浓缩得到地榆浸膏。运用大孔树脂（D101，HP-20），反相硅胶（RP-8，RP-18），Toyopearl HW-40等柱色谱方法对地榆提取物进行分离纯化，应用TLC，HPLC跟踪监测，结合重结晶技术，分离得到纯度较高的单体化合物。依据各化合物的理化常数和波谱数据分析对各单体化合物的化学结构进行进一步鉴定。结果：从地榆70%乙醇提取物中共分离得到并鉴定了13个酚酸类化合物，分别鉴定为没食子酸（1），原儿茶酸（2），3，4-二羟基苯甲醛（3），没食子酸甲酯（4），没食子酸乙酯（5），3，4-二羟基-5-甲氧基-苯甲酸甲酯（6），异落叶松脂素9'-β-D-吡喃木糖苷（7），（+）-piresil-4-O-β-D-glucopyraside（8），（+）-syringaresinol（9），1-（4-ethylphenyl）-1，2-ethanediol（10），3，4-二羟基苯甲酸乙酯（11），3，4-二甲氧基-5-羟基苯甲酸甲酯（12），4-（4-hydroxyphenyl）-2-butanone（13）。结论：除化合物1外其余12个化合物均为首次从该植物中分离得到。

Abstract

Objective: To study on chemical constituents of phenolic acids in Sanguisorbae Radix. Method: Compounds were isolated and purified by using Macroporous resin (D101

HP-20)

reversed phase silica gel (RP-8

RP-18)

Toyopearl HW-40 column chromatography and their structures were elucidated by spectral method. Result: Thirteen phenolic acid compounds were isolated from 70% ethanol extracts and their structures were elucidated as:gallic acid (1)

protocatechuic acid (2)

4-dihydroxy benzaldehyde (3)

methyl gallate (4)

ethyl gallate (5)

4-dihydroxy-5-methoxybenzoic acid methyl ester (6)

isolariciresinol 9'-β-D-xylopyranoside (7)

(+)-piresil-4-O-β-D-glucopyraside (8)

(+)-syringaresinol (9)

1-(4-ethylphenyl)-1

2-ethanediol (10)

ethyl 3

4-dihydroxy benzoate (11)

4-dimethoxy-5-hydroxybenzoic acid methyl ester (12)

and 4-(4-hydroxyphenyl)-2-butanone (13). Conclusion: Compounds 2-13 were isolated from this plant for the first time.

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