XUE Fei-fei, ZHANG Shuo-sheng, MA Jun-nan, et al. Effect of Different Processed Products of Phytolaccae Radix on Diuretic Activity and Regulation Aquaporins of Testicles and Kidneys in Rats[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(9): 1-5.
DOI:
XUE Fei-fei, ZHANG Shuo-sheng, MA Jun-nan, et al. Effect of Different Processed Products of Phytolaccae Radix on Diuretic Activity and Regulation Aquaporins of Testicles and Kidneys in Rats[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(9): 1-5. DOI: 10.13422/j.cnki.syfjx.2017090001.
Effect of Different Processed Products of Phytolaccae Radix on Diuretic Activity and Regulation Aquaporins of Testicles and Kidneys in Rats
Objective: To evaluate the diuretic effect of different processed products of Phytolaccae Radix and explore its mechanism of diuretic action. Method: A water load rat model was used
eighty SD rats were randomly divided into the blank group
model group
esculentoside A (EsA) group
raw product group and different processed products (vinegar broiled
vinegar boiled
boiled and steamed) group
10 rats in each group.At the same time
SD rats were randomly divided into the blank group
EsA group
raw product group and different processed products (vinegar broiled
vinegar boiled
boiled and steamed) group
10 rats in each group.The urine output was observed and relative mRNA expression of aquaporin-9(AQP9) of testicles
AQP2 and AQP4 of kidneys were determined. Result: In this respect of total urine output in 6 h
EsA
raw product group and different processed products of Phytolaccae Radix all showed a diuretic effect on water load rats.For normal rats
only vinegar-boiled product showed diuretic effect.PCR experiments showed that for water load rats
AQP2 mRNA expression were significantly decreased
AQP4 and AQP9 mRNA expression did not change significantly.For normal rats
AQP9 mRNA expression in vinegar-boiled group was significantly decreased
the other groups did not change significantly. Conclusion: Phytolaccae Radix for water load rats shows a diuretic effect
but it is unapparent for normal rats.Diuretic effect of Phytolaccae Radix may be related to the decrease of AQP2 and AQP9 mRNA expression.
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Related Author
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Jun-nan MA
Xiang-long MENG
Shuo-sheng ZHANG
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Related Institution
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