Objective: To evaluate in vitro transdermal absorption properties of iontophoresis fluid of Hanbi prescription for providing basis for its clinical application. Method: UV-Vis spectrophotometry was adopted to determine contents of alkaloids and glycosides with 413 nm and 232 nm as detection wavelength

respectively.Improved Franz diffusion cell was employed

permeation efficiency of different penetration enhancer combination and testing conditions of ion penetration tester on contents of alkaloids and glycosides in iontophoresis fluid were evaluated. Result: The optimized combination of permeation enhancers was 1.2 mL of azone

1.6 g of sodium dodecyl sulfate

0.4 mL of methyl pyrrolidone

0.4 mL of propylene glycol and 1.2 mL of oleic acid;under these conditions

the average cumulative osmotic quantity of alkaloids and glycosides from iontophoresis fluid of Hanbi prescription in 24 h were 15.84

1 379.82 μg·cm-2

their increased permeation times were 1.77-fold and 2.30-fold over that without permeation enhancer

respectively.When the voltage of ion penetration tester was 2.4 V

the penetration rates of alkaloids and glycosides in iontophoresis fluid of Hanbi prescription were 20.74% and 1.24% in 30 min

and 4.20-fold and 5.17-fold over that without the permeation enhancers and iontophoresis. Conclusion: Iontophoresis can significantly improve transdermal absorption of alkaloids and glycosides in iontophoresis fluid of Hanbi prescription across skin

and it will be benefit for curing Hanbi symptom.