Objective: To analyze the endogenous metabolite changes in the fecal supernatant of mice infected with H1N1 influenza virus and intervened by Mahuang Xixin Fuzi Tang (MXF)

and to explore the therapeutic mechanism of MXF through metabolomics technology. Method: KM mices were randomly divided into blank group

model group and MXF group

modle mice were intranasally inoculated with H1N1 influenza virus

and then intervened by MXF (dose of 20 mL·kg-1) through orally administration.All drug treatments were administered daily for consecutive 7 days

body weight and rectal temperature were measured

the feces were collected.Ultra performance liquid chromatography coupled to Q-exactive high resolution mass spectrometry analysis was prformed on a Halo C18 column (2.1 mm×100 mm

2.7 μm) with 0.05% formic acid in water and 0.05% formic acid in acetonitrile for gradient elution as mobile phase and the mass spectral scanning range of m/z 80-1 200.LC-MS data were processed with principal components analysis (PCA) and partial least squares discriminant analysis (PLS-DA) to find out potential biomarkers. Result: Significant differences were found in the metabolic profiles among the blank group

model group and MXF group.Nine metabolites were identified as potential biomarkers

such as phosphatidylethanolamine

phosphatidylserine

phosphatidylcholine

lyso-phosphatidylcholine

L-kynurenine

oxalacetic acid

pyridoxal

succinic acid and melatonin. Conclusion: The flu induced by H1N1 virus may interfere with the typophan

vitamin B6

glycerol phospholipids and tricarboxylic acid cycle metabolic pathway according to these identified biomarkers.MXF may play a therapeutic role in a way of regulating the above disordered metabolic pathways.