Objective: To investigate the resolvability of chemical constituents in Phellodendri Amurensis Cortex and their effects on heat syndrome rats. Method: We split the chemical constituents in Phellodendri Amurensis Cortex with 95% ethanol extraction

ethyl acetate extraction

macroporous resin and cation exchange resin separation.We established the total ion current fingerprint of these chemical constituents by UPLC-Q-TOF/MS for principal component analysis (PCA).We observed effect of the spilt components in Phellodendri Amurensis Cortex on central nervous system neurotransmitter and energy metabolism of heat syndrome rats induced by levothyroxine sodium tablets. Result: The chemical constituents in Phellodendri Amurensis Cortex were split three components

namely

alkaloid component

non-alkaloid component and ethyl acetate component

their yields were 0.65%

4.70% and 2.50%.The chemical components in Phellodendri Amurensis Cortex were proved basically no cross by PCA.The chemical components in Phellodendri Amurensis Cortex should decrease the contents of neurotransmitters

hormones and enzymes related to central neurotransmitters and energy metabolism in the serum and liver of heat syndrome rats with different degrees. Conclusion: The material basis of ‘cold’ nature of Phellodendri Amurensis Cortex are mianly for alkaloid component and ethyl acetate component.