Accumulation of Main Secondary Metabolites in Leaves and Stems of  var. with Multi-stem

WANG Cai-bu; HAN Duo; YANG Min; LI Hai-feng

doi:10.13422/j.cnki.syfjx.2017100034

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Accumulation of Main Secondary Metabolites in Leaves and Stems of var. with Multi-stem

更新时间：2024-01-04

- Accumulation of Main Secondary Metabolites in Leaves and Stems of var. with Multi-stem
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 10, Pages: 34-38(2017)
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- DOI：10.13422/j.cnki.syfjx.2017100034
  CLC： S567.239
- Published：2017
- 稿件说明：
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WANG Cai-bu, HAN Duo, YANG Min, et al. Accumulation of Main Secondary Metabolites in Leaves and Stems of var. with Multi-stem[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(10): 34-38.
DOI：

WANG Cai-bu, HAN Duo, YANG Min, et al. Accumulation of Main Secondary Metabolites in Leaves and Stems of var. with Multi-stem[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(10): 34-38. DOI： 10.13422/j.cnki.syfjx.2017100034.

摘要

目的：探讨不同居群多茎滇重楼叶和茎中主要次生代谢产物的积累规律。方法：采用HPLC测定不同居群多茎滇重楼叶和茎中主要活性成分重楼皂苷Ⅰ，Ⅱ，Ⅵ，Ⅶ的含量，流动相水（A）-乙腈（B）梯度洗脱（0~40 min，30%~60%B；40~50 min，60%~30%B），检测波长203 nm，运用单因素方差分析、聚类分析和HPLC图谱分析探讨其次生代谢产物的积累规律。结果：不同居群多茎滇重楼叶中重楼皂苷Ⅱ，Ⅵ，Ⅶ含量及总含量具有显著差异；除个别居群外，不同居群多茎滇重楼茎中重楼皂苷Ⅶ含量差异较小。叶中主要检测到重楼皂苷Ⅱ和Ⅶ，茎中只检测到重楼皂苷Ⅶ。多茎滇重楼和滇重楼比较的地上部分叶和茎HPLC图谱相似度均较低，6个居群叶中重楼皂苷Ⅰ，Ⅱ，Ⅵ，Ⅶ总质量分数达到了2015年版《中国药典》规定的限量标准。结论：多茎滇重楼与滇重楼地上部分叶和茎存在一定差异，但多茎滇重楼叶中有效物质成分较高，该研究为多茎滇重楼地上部分叶和茎的开发利用提供了一定的参考依据。

Abstract

Objective: To evaluate the accumulation of main secondary metabolites in leaves and stems of Paris polyphylla var. yunnanensis with multi-stem. Method: The contents of polyphyllin Ⅰ

Ⅱ

Ⅵ and Ⅶ in the leaves and stems of P. polyphylla var. yunnanensis with multi-stem were determined by HPLC.One-way ANOVA

cluster analysis and HPLC analysis were used to evaluate the accumulation of its secondary metabolites. Result: There were significant differences in the contents of polyphyllin Ⅱ

Ⅵ

Ⅶ and their total content in the leaves of P. polyphylla var. yunnanensis with multi-stem;in addition to individual populations

polyphyllin Ⅶ content had a certain difference in the stems of P. polyphylla var. yunnanensis with multi-stem.Polyphyllin Ⅱ and polyphyllin Ⅶ were mainly detected in the leaves

and only polyphyllin Ⅶ was detected in the stems.The similarity of HPLC were low both in the leaves and stems of P. polyphylla var. yunnanensis with multi-stem and P. polyphylla var. yunnanensis.Six populations of P. polyphylla var. yunnanensis with multi-stem had reached the standard of the 2015 edition of Chinese Pharmacopoeia in the total content of polyphyllin Ⅰ

Ⅱ

Ⅵ and Ⅶ. Conclusion: There are some differences in accumulation of main secondary metabolites in the leaves and stems between P. polyphylla var. yunnanensis with multi-stem and P. polyphylla var. yunnanensis

but the contents of active substances is higher in the former.This study provides a scientific basis for development and utilization of P. polyphylla var. yunnanensis with multi-stem.

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