WU Jin-feng, WANG Xue, PAN You-zhi, et al. Determination of Active Constituents in Congrong Zonggan Capsule by UPLC and QAMS[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(10): 44-49.
DOI:
WU Jin-feng, WANG Xue, PAN You-zhi, et al. Determination of Active Constituents in Congrong Zonggan Capsule by UPLC and QAMS[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(10): 44-49. DOI: 10.13422/j.cnki.syfjx.2017100044.
Determination of Active Constituents in Congrong Zonggan Capsule by UPLC and QAMS
Objective: To establish a quantitative analysis for the determination of the six components in Congrong Zonggan capsule based on multi-components with a single-marker (QAMS) method and ultra-high performance liquid chromatography (UPLC)
and compare with the standard curve method
in order to evaluate the adaptation and application of QAMS method in the quality control of Congrong Zonggan capsule. Method: Chrlorogenic acid and acteoside were used as the internal reference substances. The relative correlation factors (RCF) of caffeic acid with chrlorogenic acid
as well as tubuloside A
isoacteoside and echinacoside with acteoside were calculated and established. At the same time
the durability of each RCF was investigated. The results were compared with those obtained by the standard curve method
in order to verify the accuracy and feasibility of the method. Result: Within the linear ranges
the RCF values of chlorogenic acid with caffeic acid was 0.538
and that of acteoside with tubuloside A
isoacteoside and echinacoside were 1.346
1.266 and 1.135
respectively. The durability of each RCF was good. The two methods showed no significant difference in assay results for the 10 batches of samples. The obtained RCF was credible. Conclusion: The QAMS method is feasible and credible
and could be used to determine the multiple components in Congrong Zonggan capsule.
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Related Author
ZHANG Yongxiang
ZHANG Bao
LIN Lianghua
YANG Fan
XUE Shujuan
GU Li
ZHANG Zhongyi
ZHANG Liuji
Related Institution
School of Pharmacy, Henan University of Chinese Medicine
College of Agriculture, Fujian Agriculture and Forestry University
Key Laboratory of Geoherbalism and Quality Evaluation Methods of Four Major Huai Medicines of National Administration of Traditional Chinese Medicine, Henan Integrative Medicine Hospital of Institute of Chinese Materia Medica
Key Laboratory of Geoherbalism and Quality Evaluation Methods of Four Major Huai Medicines of the National Administration of Traditional Chinese Medicine, Henan Academy of Chinese Medicine