Determination of 6 Different Alkaloids in Processed Aconiti Lateralis Radix from Fuzi Lizhong Wan

SONG Zhi-qian; GAN Jia-he; DONG Yun-zhuo; WANG Chun; NING Zhang-chi; LIU Zhen-li

doi:10.13422/j.cnki.syfjx.2017100055

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Determination of 6 Different Alkaloids in Processed Aconiti Lateralis Radix from Fuzi Lizhong Wan

更新时间：2024-01-04

- Determination of 6 Different Alkaloids in Processed Aconiti Lateralis Radix from Fuzi Lizhong Wan
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 10, Pages: 55-60(2017)
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- DOI：10.13422/j.cnki.syfjx.2017100055
  CLC： R286.0
- Published：2017
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SONG Zhi-qian, GAN Jia-he, DONG Yun-zhuo, et al. Determination of 6 Different Alkaloids in Processed Aconiti Lateralis Radix from Fuzi Lizhong Wan[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(10): 55-60.
DOI：

SONG Zhi-qian, GAN Jia-he, DONG Yun-zhuo, et al. Determination of 6 Different Alkaloids in Processed Aconiti Lateralis Radix from Fuzi Lizhong Wan[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(10): 55-60. DOI： 10.13422/j.cnki.syfjx.2017100055.

摘要

目的：建立高效液相色谱-三重四级杆串联质谱（HPLC-QQQ-MS）法同时测定附子理中丸中制附子6种生物碱成分含量的方法。方法：采用Thermo Accucore RP-MS色谱柱（2.1 mm×100 mm，2.6 μm），以0.1%甲酸水-0.1%甲酸乙腈为流动相，流速0.3 mL·min-1，柱温30 ℃。质谱条件：采用电喷雾电离源（ESI），正离子检测模式，扫描方式为多反应离子监测模式（MRM），定量分析离子分别为苯甲酰新乌头碱m/z 105.0，苯甲酰乌头碱m/z 105.0，苯甲酰次乌头碱m/z 542.3，新乌头碱m/z 572.3，乌头碱m/z 586.3，次乌头碱m/z 556.3。结果：苯甲酰新乌头碱、苯甲酰乌头碱、苯甲酰次乌头碱、新乌头碱、乌头碱、次乌头碱线性范围分别在0.184 80~1.848 ng（r=0.999 6），0.035 36~0.353 6 ng（r=0.999 6），0.129 60~1.296 ng（r=0.999 8），0.011 65~0.116 5 ng（r=0.999 8），0.002 95~0.029 5 ng（r=0.999 8），0.036 96~0.369 6 ng（r=0.999 8），6种生物碱的含量在一定范围内线性关系良好（r> 0.999 6）。加样回收率在91.93%~106.70%，RSD均<6.00%。结论：该方法简单、准确、灵敏、重复性好，可用于附子理中丸中制附子生物碱成分的含量测定。

Abstract

Objective: To establish a HPLC-QQQ-MS method for simultaneous determination of six alkaloids in processed Aconiti Lateralis Radix Praeparaia from Fuzi Lizhong Wan. Method: Thermo Accucore column (2.1 mm×100 mm

2.6 μm) was adopted and eluted with mobile phase of 0.1% formic acid in water-0.1% formic acid in acetonitrile

with a flow rate at 0.3 mL·min-1. The column temperature was set at 30 ℃. The detection was accomplished in the positive ionization mode with scanning viaelectrospray ionization (ESI)

the scanning mode was multiple-reaction monitoring (MRM). According to the quantitative analysis

the ions were benzoylmesaconine m/z 105.0

benzoylaconitine m/z 105.0

benzoylhypaconitine m/z 542.3

mesaconitine m/z 572.3

aconitine m/z 586.3 and hypaconitine m/z 556.3. Result: Benzoylmesaconine

benzoylaconitine

benzoylhypaconitine

mesaconitine

aconitine and hypaconitine had a good linearity in the ranges of 0.184 80-1.848 ng (r=0.999 6)

0.035 36-0.353 6 ng (r=0.999 6)

0.129 60-1.296 0 ng (r=0.999 8)

0.011 65-0.116 5 ng (r=0.999 8)

0.002 95-0.029 5 ng (r=0.999 8)

0.036 96-0.369 6 ng (r=0.999 8)

respectively. The recoveries for the six analytes were between 91.93% and 106.70%

with RSD less than 6%. Conclusion: This method is simple

accurate

sensitive and repeatable

and can be used to determine content of alkaloids in processed Aconiti Lateralis Radix praeparaia from Fuzi Lizhong Wan.

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