Objective: To establish a GC chromatographic fingerprint analysis method of volatile components in Tibetan medicine Zhenzhu pills (Ershiwuwei Zhenzhu Wan and Qishiwei Zhenzhu pills)

initially determine the properties of their part components

and provide experimental basis for quality control. Method: Agilent DB-1 capillary column (0.25 mm×30 m

0.25 μm) was used. The injection port temperature was 200 ℃; while the FID detector temperature was 320 ℃. The temperature program was as follows: the initial column temperature was 60 ℃

maintained for 1 min; then 4 ℃·min-1 up to 90 ℃

maintained for 1 min; 2 ℃·min-1 up to 110 ℃

maintained for 10 min; 2.5 ℃·min-1 up to 200 ℃

maintained for 5 min; and finally 5 ℃·min-1 up to 280 ℃

maintained for 8 min. The carrier gas was nitrogen and its flow rate was 3 mL·min-1

with a diversion ratio of 5:1. Result: In all common peaks in the GC characteristic chromatogram

all of their precision

stability and repeatability met the requirements (RSD< 5.0%). Analysis of similarity showed small differences in the chemical compositions from various batches of samples

but the difference in the contents and proportion of chemical compositions were obvious. Conclusion: The established GC chromatographic fingerprint method was in good separation

and can be used as a quality control method for Tibetan medicine Zhenzhu pills.