Objective: To clarify the anti-ulcer chemical compositions of bupleurum saponins and explore the pharmacokinetic-pharmacodynamic relationship of bupleurum saponins for curing gastric ulcer

which could lay an experimental basis for exploring its effective substance and mechanism action. Method: High resolution mass spectrometry

first and secondary mass spectrometry method were used to explore the chemical compositions of bupleurum saponins

and at the same time

the blood of SD rats on different time points after orally administration of bupleurum saponins extract (300 mg·kg-1) was taken. HPLC method was used to determinate the blood drug concentration of bupleurum saponins B2 and bupleurum saponins D; the time-effectiveness correlation between bupleurum saponins B2 and D concentrations and the proliferation rate of human gastric epithelial cells in vitro

superoxide dismutase (SOD) and malondialdehyde (MDA) levels was analyzed; and the characteristics of effect and the possible mechanism of bupleurum saponins were evaluated. Result: The main anti-ulcer chemical compositions of bupleurum saponins were saikosaponin A

D

B2

I

E

prosaikogenin D

F

and G. Bupleurum saponins drug-containing plasma and the proliferation rate of human gastric mucosa epithelial cells presented a good correlation

showing a certain time-effect relationship with MDA and SOD levels in blood. Conclusion: This study has clarified the anti-ulcer chemical compositions of bupleurum saponins

explored the correlation between the components absorbed in blood and the efficacy

and its mechanism may be associated with MDA

SOD protein regulation.