Mechanism of Flavonoids from  Leaves in Promoting Pancreatic Islets Regeneration in Mouse with Diabetes Meilitus

LI Jie; LI Dong-hua; TU Zheng-wei; FU Yu; LIU Hong-bin; ZHANG Yi; ZONG Chun-hui; YU Qiang

doi:10.13422/j.cnki.syfjx.2017100116

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Mechanism of Flavonoids from Leaves in Promoting Pancreatic Islets Regeneration in Mouse with Diabetes Meilitus

更新时间：2024-01-04

- Mechanism of Flavonoids from Leaves in Promoting Pancreatic Islets Regeneration in Mouse with Diabetes Meilitus
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 10, Pages: 116-121(2017)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2017100116
  CLC： R-332;R285.5
- Published：2017
- 稿件说明：
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LI Jie, LI Dong-hua, TU Zheng-wei, et al. Mechanism of Flavonoids from Leaves in Promoting Pancreatic Islets Regeneration in Mouse with Diabetes Meilitus[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(10): 116-121.
DOI：

LI Jie, LI Dong-hua, TU Zheng-wei, et al. Mechanism of Flavonoids from Leaves in Promoting Pancreatic Islets Regeneration in Mouse with Diabetes Meilitus[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(10): 116-121. DOI： 10.13422/j.cnki.syfjx.2017100116.

摘要

目的：观察番石榴叶总黄酮对链脲佐菌素（streptozocin，STZ）糖尿病模型小鼠胰腺组织中胰腺十二指肠同源盒因子-1（pancreas-duodenumhomeobox-1，PDX-1），神经元素3（neurogenin 3，Ngn3），NK6转录因子相关基因座1（NK6 transcription factor related locus 1，Nkx6.1）表达的影响，探讨其促进胰岛β细胞再生的机制。方法：将STZ糖尿病模型小鼠随机分为模型组和治疗组，治疗组包括番石榴叶总黄酮低、高剂量组及二甲双胍组，另设10只正常小鼠为正常组。番石榴叶总黄酮低、高剂量组按小鼠体重分别以番石榴叶总黄酮0.198，0.396 g·kg-1·d-1灌胃，二甲双胍组以二甲双胍0.087 5 g·kg-1·d-1灌胃，正常组和模型组以等体积蒸馏水灌胃，2周后检测其空腹血糖、体重，苏木素-伊红（HE）染色观察胰岛病理形态学改变，实时荧光定量聚合酶链反应（Real-time PCR）检测胰腺组织内胚胎期胰岛β细胞发育相关转录因子PDX-1，Ngn3，Nkx6.1 mRNA相对表达量。结果：与正常组比较，STZ糖尿病模型小鼠胰腺组织中PDX-1，Ngn3，Nkx6.1表达量明显下降（P<0.01）；与模型组比较，番石榴叶总黄酮治疗组PDX-1，Ngn3，Nkx6.1 mRNA表达升高（P<0. 05）。结论：番石榴叶总黄酮能上调糖尿病小鼠胰腺组织中PDX-1，Ngn3，Nkx6.1的表达；胰岛β细胞再生机制可能与PDX-1，Ngn3，Nkx6.1 mRNA表达的上调有关。

Abstract

Objective: To investigate the effect of flavonoids from Psidium guajava leaves on pancreatic duodenum homeobox-1 (PDX-1)

neurogenin 3 (Ngn3)

NK6 transcription factor-related locus 1 (Nkx6.1) expression in pancreas of streptozotocin (STZ)-induced diabetic mice

and explore the mechanism of pancreatic islets regeneration. Method: Diabetic mouse were randomly divided into model group and treatment groups

the latter included P. guajava leaves flavonoids low and high-dose groups and metformin group

and the other 10 normal mice were included into normal group. P. guajava leaves flavonoids low and high-dose groups were administered intragastrically with the doses of 0.198

0.396 g·kg-1·d-1

metformin was 0.087 5 g·kg-1·d-1

and normal group and model group were administered intragastrically with the same volume of distilled water. After two weeks of administration

fasting blood glucose and weight were measured. Pancreatic tissue was sliced

and observed by hematoxylin-eosin (HE) staining. And Real-time PCR was used to investigate the mRNA expressions of PDX-1

Ngn3

Nkx6.1. Result: Compared with normal group

the expressions of PDX-1

Ngn3 and Nkx6.1 were decreased in pancreas of STZ-induced diabetic mice (P<0. 05). Compared with model group

the expressions of PDX-1

Ngn3 and Nkx6.1 were increased in P. guajava leaves flavonoids low and high-dose groups (P<0.05). Conclusion: P. guajava leaves flavonoids can increase the expressions of PDX-1

Ngn3

Nkx6.1 in the pancreatic tissues in mouse with diabetes mellitus. Its mechanism of pancreatic islets β cell regeneration may be related to the increase in expressions of PDX-1

Ngn3

Nkx6.1.

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