Discussion on Mechanism of Attenuating Toxicity and Saving Effect of  Compatibility with Processed Aconiti Lateralis Radix Praeparata and Zingiberis Rhizoma Based on ‘Fuyang Buxu’ Theory

ZHANG Yue-chan; WANG Jian-chun; PAN Jin-huo; ZHANG Jing; DU Jia; WANG Wen-yi

doi:10.13422/j.cnki.syfjx.2017110007

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Discussion on Mechanism of Attenuating Toxicity and Saving Effect of Compatibility with Processed Aconiti Lateralis Radix Praeparata and Zingiberis Rhizoma Based on ‘Fuyang Buxu’ Theory

更新时间：2024-01-04

- Discussion on Mechanism of Attenuating Toxicity and Saving Effect of Compatibility with Processed Aconiti Lateralis Radix Praeparata and Zingiberis Rhizoma Based on ‘Fuyang Buxu’ Theory
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 11, Pages: 7-12(2017)
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- DOI：10.13422/j.cnki.syfjx.2017110007
  CLC： R283
- Published：2017
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ZHANG Yue-chan, WANG Jian-chun, PAN Jin-huo, et al. Discussion on Mechanism of Attenuating Toxicity and Saving Effect of Compatibility with Processed Aconiti Lateralis Radix Praeparata and Zingiberis Rhizoma Based on ‘Fuyang Buxu’ Theory[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(11): 7-12.
DOI：

ZHANG Yue-chan, WANG Jian-chun, PAN Jin-huo, et al. Discussion on Mechanism of Attenuating Toxicity and Saving Effect of Compatibility with Processed Aconiti Lateralis Radix Praeparata and Zingiberis Rhizoma Based on ‘Fuyang Buxu’ Theory[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(11): 7-12. DOI： 10.13422/j.cnki.syfjx.2017110007.

摘要

目的：研究炮附子、干姜配伍关木通对马兜铃酸A含量的影响，对大鼠利尿作用、急性肾功能损伤的影响，以及对体外肾细胞毒性的影响。方法：根据中医药理论，选取不同比例炮附子、干姜配伍关木通，通过HPLC比较配伍前后关木通中马兜铃酸A的含量变化，流动相乙腈-0.4%冰乙酸水溶液（42：58），检测波长318 nm；通过大鼠水负荷动物模型比较配伍前后关木通的利尿作用变化；通过大鼠急性肾损伤试验比较配伍前后大鼠急性肾功能损伤的变化；通过倒置显微镜观察细胞形态，利用CCK-8检测细胞活力，比较配伍前后关木通对人肾小管上皮细胞毒性的变化。结果：炮附子-干姜-关木通（3：2：1）配伍使用时关木通中马兜铃酸A显著降低，降低率达19.18%，而对大鼠的利尿作用没有影响，对大鼠急性肾功能损伤明显低于关木通生品，细胞活力优于关木通生品。结论：炮附子、干姜配伍关木通可以达到一定的减毒存效作用，对解决含马兜铃酸A的中药饮片现有临床应用问题具有一定的指导意义。

Abstract

Objective: To discuss effect of compatibility of Aristolochia manshuriensis with processed Aconiti Lateralis Radix Praeparata and Zingiberis Rhizoma on the content of aristolochic acid A

diuretic action of rats

acute renal injury of rats and in vitro renal cytotoxicity. Method: Different proportion of processed Aconiti Lateralis Radix Praeparata and Zingiberis Rhizoma compatibility with A. manshuriensis was chosen according the theory of traditional Chinese medicine (TCM).The content of aristolochic acid A after compatibility was determined by HPLC.The change of diuretic effect after compatibility was compared by rat water-load test

the change of rat acute renal injury after compatibility was compared

the change of human renal tububar epithelial cell toxicity after compatibility was compared inverted microscope observation and CCK-8. Result: After compatibility of processed Aconiti Lateralis Radix Praeparata-Zingiberis Rhizoma-A. manshuriensis (3: 2: 1)

the content of aristolochic acid A depressed markedly with reduction percentage of 19.18%

diuretic effect of A. manshuriensis on rats had no change

its acute renal injury in rats was significantly lower than the crude product

its cell activity was better than the crude product. Conclusion: Compatibility can reduce the toxicity and save the effect of A. manshuriensis

it has a certain guiding significance to solve current clinical applications of TCM containing aristolochic acid A.

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