Comparison on Precise Powder Decoction Pieces and Traditional Commercial Slices：A Case Study on Salviae Miltiorrhizae Radix et Rhizoma

HUANG Juan; QIU Xiao-hui; BAI Jun-qi; GONG Lu; ZHANG Peng; SU He; XU Jiang; HUANG Zhi-hai

doi:10.13422/j.cnki.syfjx.2017110018

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Comparison on Precise Powder Decoction Pieces and Traditional Commercial Slices：A Case Study on Salviae Miltiorrhizae Radix et Rhizoma

更新时间：2024-01-04

- Comparison on Precise Powder Decoction Pieces and Traditional Commercial Slices：A Case Study on Salviae Miltiorrhizae Radix et Rhizoma
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 11, Pages: 18-22(2017)
- 作者机构：
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- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2017110018
  CLC： R284.1
- Published：2017
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HUANG Juan, QIU Xiao-hui, BAI Jun-qi, et al. Comparison on Precise Powder Decoction Pieces and Traditional Commercial Slices：A Case Study on Salviae Miltiorrhizae Radix et Rhizoma[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(11): 18-22.
DOI：

HUANG Juan, QIU Xiao-hui, BAI Jun-qi, et al. Comparison on Precise Powder Decoction Pieces and Traditional Commercial Slices：A Case Study on Salviae Miltiorrhizae Radix et Rhizoma[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(11): 18-22. DOI： 10.13422/j.cnki.syfjx.2017110018.

摘要

目的：对丹参精准煮散饮片进行质量评价，为临床用药安全有效提供保障。方法：应用第二内转录间隔区（ITS2）序列作为DNA条形码对丹参进行鉴定，采用化学指纹图谱表征药材化学组成，评价丹参原饮片及精准煮散饮片的相似度，测定指标成分丹酚酸B和迷迭香酸的含量，标定指纹图谱共有峰。采用标准汤剂煎煮法比较原饮片及精准煮散饮片的煎煮效率，对煮散体系进行综合评价。结果：ITS2序列对丹参药材可实现准确鉴定；原饮片及煮散饮片煎出成分基本无变化，指纹图谱相似度均很高，达到0.99以上，但是煮散饮片煎煮效率明显增高。按标准汤剂煎煮法，煮散饮片出膏率增加>30%，迷迭香酸及丹酚酸B的煎出率均约1.7倍，其余化学成分的煎出率为原饮片的1.2~2.2倍；且精准煮散饮片差异明显小于原饮片批间差异。结论：丹参精准煮散饮片能提高原饮片的煎煮效率及均一性，具有较好的推广与应用价值。

Abstract

Objective: To investigate the rationality and superiority of precise powder decoction pieces system

Salviae Miltiorrhizae Radix et Rhizoma was employed in this study. Method: The second internal transcribed spacer (ITS2) sequence was used as a DNA barcode to identify Salviae Miltiorrhizae Radix et Rhizoma.The chemical composition of the medicinal materials was characterized by HPLC fingerprints

similarity between precise powder decoction pieces and traditional commercial slices were both evaluated.The concents of salvianolic acid B and rosmarinic acid were determined by HPLC

and the characteristic common peaks were identified.In addition

the extraction efficiency was also compared by standard decoction method. Result: Salviae Miltiorrhizae Radix et Rhizoma could be accurately identified by ITS2 sequence.HPLC fingerprints were very similarity between precise powder decoction pieces and traditional commercial slices.However

the extraction efficiency of precise powder decoction pieces was significantly improved with >30% increased dry extract yield and approximately 20%-120% increased dissolution of chemical components.Moreover

the relative standard deviation was also decreased between batches. Conclusion: Precise powder decoction pieces can improve the extraction efficiency and uniformity of traditional commercial slices.

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