Objective: By measuring dissolution contents of three kinds of toxic components (triptolide，tripterine

and wilforlide) in simplified Qingluo Tongbi decoction

Tripterygium wilfordii(TW)

TW +Notoginseng Radix et Rhizoma

as well as TW+Rehmanniae Radix

explore ‘Diversity Restriction’ mechanism of detoxicity from the perspective of the chemical basis composition

and provide experimental basis for clinical compatibility application of TW for detoxicity effect. Method: Highly selective and sensitive triple quadrupole mass spectrometry (LC-MS/MS) were used at the same time to detect three active ingredients-triptolide

tripterine and wilforlide

and then their dissolution changes were compared between TW

TW+ Notoginseng Radix et Rhizoma

TW+Rehmanniae Radix

as well as Simplified Qingluo Tongbi decoction. Result: In TW medicinal water extract

triptolide had the highest content

followed by wilforlide

and the content of tripterine was lowest. When the amount of medicine TW was same

the dissolution trend of the above three active ingredients was consistent under different compatibility environments

and the dissolution order was as follows: TW> TW+Rehmanniae Radix> TW+Notoginseng Radix et Rhizoma >Simplified Qingluo Tongbi decoction. Conclusion: In the compatibility

Notoginseng Radix et Rhizoma had a significant inhibitory effect on dissolution of three active ingredients of TW

while Rehmanniae Radix had a weak inhibitory effect. In addition

the dissolution of three active ingredients was significantly reduced in Simplified Qingluo Tongbi decoction. The results provided an experimental basis for clinical ‘Diversity Restriction’ detoxicity mechanism of TW.