Determination of Two Saponins in  Leaves from Different Habitats and Gathering Periods

LI Bai-mei; SUN Yi-chun; WANG Qiu-hong; WANG Zhi-bin; KUANG Hai-xue

doi:10.13422/j.cnki.syfjx.2017110059

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Determination of Two Saponins in Leaves from Different Habitats and Gathering Periods

更新时间：2024-01-04

- Determination of Two Saponins in Leaves from Different Habitats and Gathering Periods
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 23, Issue 11, Pages: 59-62(2017)
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2017110059
  CLC： R284.1
- Published：2017
- 稿件说明：
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LI Bai-mei, SUN Yi-chun, WANG Qiu-hong, et al. Determination of Two Saponins in Leaves from Different Habitats and Gathering Periods[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(11): 59-62.
DOI：

LI Bai-mei, SUN Yi-chun, WANG Qiu-hong, et al. Determination of Two Saponins in Leaves from Different Habitats and Gathering Periods[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(11): 59-62. DOI： 10.13422/j.cnki.syfjx.2017110059.

摘要

目的：测定并比较不同产地和不同采收期辽东楤木叶中3-O-β-D-葡萄吡喃糖基-（1→3）-β-D-葡萄吡喃糖基-（1→3）-β-D-葡萄吡喃糖基-齐墩果酸-28-O-β-D-吡喃葡萄糖苷（楤木皂苷TTP）和3-O-[β-D-葡萄吡喃糖基（1→2）]-[β-D-葡萄吡喃糖基（1→3）]-β-D-葡萄吡喃糖基-齐墩果酸-28-O-β-D-葡萄吡喃糖酯（楤木皂苷V）的含量。方法：采用高效液相色谱蒸发光散射检测（HPLC-ELSD）法，使用Diamonsil C18（2）色谱柱（4.6 mm×250 mm，5 μm），流动相乙腈-水（31：69），流速1.0 mL·min-1，柱温30 ℃，蒸发光散射检测器漂移管温度110 ℃，载气（N2），流速3.2 mL·min-1。结果：楤木皂苷TTP和楤木皂苷V分别在进样量0.51~2.55 μg和0.50~4.00 μg线性关系良好，相关系数分别为0.999 8和0.999 2。结果表明，不同产地和不同采收期辽东楤木叶中楤木皂苷TTP和楤木皂苷V的含量有所差异，可能与辽东楤木分布区域、生长季节及叶片生理状态有关，由实验结果可以看出，两种皂苷类成分大多在7，8月的含量相对较高，部分地区9月含量较高。结论：该方法简单、快捷、准确，可以同时测定辽东楤木叶中楤木皂苷TTP和楤木皂苷V的含量，为辽东楤木叶质量控制提供实验依据。

Abstract

Objective: To determine and compare the contents of congmunoside TTP and congmunoside V in Aralia elata leaves from different habitats and gathering periods. Method: HPLC-ELSD was carried out on a Diamonsil C18 (2) column (4.6 mm×250 mm

5 μm) at a column temperature of 30 ℃. The mobile phase was acetonitrile and water (31: 69) at flow rate of 1.0 mL·min-1. The detector was ELSD

with the temperature of drift tube set at 110 ℃

and the flow rate of carrier gas (N2) was 3.2 mL·min-1. Result: The linear ranges of congmunoside TTP and congmunoside V were 0.51-2.55 μg (r=0.999 8) and 0.50-4.00 μg (r=0.999 2)

respectively. According to the results

the contents of congmunoside TTP and congmunoside V in A. elata leaves from different habitats and gathering periods were different. This may be related to distribution areas

growing seasons and physiological state of leaves. On the basis of the experimental results

the content of two kinds of ingredients was high mostly in July and August

and partially in September. Conclusion: The method is simple

rapid and accurate for determining simultaneously the contents of congmunoside TTP and congmunoside V in A. elata leaves

and can provide a basis for the quality control of A. elata leaves.

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