Objective: To establish a UPLC method for the simultaneous determination of eight compounds in Forsythiae Fructus from different habitats. Method: Eight compounds in Forsythiae Fructus were simultaneously determined by UPLC with BEH C18 column (2.1 mm×50 mm

1.7 μm)

with methanol (A)-water (B) (consisting of 0.1%formic acid) as the mobile phase for gradient elution (0-2 min

10%-20%A; 2-3 min

20%-30%A; 3-6 min

30%-60%A; 6-6.01 min

60%-10%A; 6.01-9 min

10%A); at the flow rate of 0.4 mL·min-1; the determination wavelength was 235 nm; and the injection volume was 2 μL. Result: Eight components were separated clearly within 6 min. The RSD values of precision

stability and reproducibility were all less than 3.0%. The average recoveries were 98.8%-101.4%with RSD of 1.0%-1.9%. Conclusion: The method is simple

rapid

accurate and highly sensitive

providing a scientific basis for quality assessment of Forsythiae Fructus. PCA results showed that different production place

harvesting seasons and processing method played an important role in quality of Forsythiae Fructus.