Objective: To establish a HPLC method to simultaneously determine the content of caffeic acid

acteoside

isoacteoside and pogostone in Pogostemonis Herba formula granules

in order to provide basis for studying its quality standards. Method: An HPLC method was developed. Wondasil C18 column (4.6 mm×250 mm

5 μm) was used and eluted with mobile gradient of 0.1% phosphoric acid-acetonitrile at the flow rate of 1.0 mL·min-1. The wavelength were set at 325 nm (0-18 min

caffeic acid)

330 nm (18-30 min

acteoside and isoacteoside)

and 311 nm (30-55 min

pogostone). The column temperature was 30 ℃. Result: The good linear relationships between the concentration and peak area were between 0.021 8-0.218 μg (r=0.999 8) for caffeic acid

0.032 6-0.326 μg (r=0.999 8) for acteoside

0.099 4-0.994 μg (r=0.999 7) for isoacteoside acid and 0.174 2-1.742 μg for pogostone (r=0.999 9)

respectively. The four tested indicators of sample solutions were steady within 24 h. The average recoveries were 98.85%

99.53%

99.37%

100.58%

with RSD of 1.9%

1.1%

1.4%

2.0%

respectively. Conclusion: Caffeic acid

acteoside

isoacteoside and pogostone were determined by this method simultaneously. The method is feasible and accurate with a good reproducibility

and can provide a reference for quality control.