GENG Ting, ZHANG She-bing, LI Yan-jing, et al. Analysis of Metabolites of Ginkgo Diterpene Lactone Meglumine Injection in Human Urine by LC-MS/MS[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(11): 90-95.
DOI:
GENG Ting, ZHANG She-bing, LI Yan-jing, et al. Analysis of Metabolites of Ginkgo Diterpene Lactone Meglumine Injection in Human Urine by LC-MS/MS[J]. Chinese journal of experimental traditional medical formulae, 2017, 23(11): 90-95. DOI: 10.13422/j.cnki.syfjx.2017110090.
Analysis of Metabolites of Ginkgo Diterpene Lactone Meglumine Injection in Human Urine by LC-MS/MS
Objective: To investigate the major metabolites of ginkgolide A (GA)
ginkgolide B (GB) and ginkgolide K (GK) from ginkgo diterpene lactone meglumine injection in human urine. Method: The urine samples of the volunteers after a single intravenous infusion administration of ginkgo diterpene lactone meglumine injection were collected for 0-6 h
then the samples were prepared with or without acidification treatment and analyzed by LC-MS/MS.The potential metabolites of GA
GB and GK were analyzed through comparing the total ion current (TIC) and extract ion current (EIC) chromatogram of the test samples with the blank urine and analyzing the quasi-molecular ion and secondary fragment ion of all chromatograms. Result: In addition to the prototype drugs
twelve metabolites were found in human urine containing five phase Ⅰ metabolites and one phase Ⅱ metabolite from GA
one phase Ⅰ metabolite and three phase Ⅱ metabolites from GB
one phase Ⅰ metabolite and one phase Ⅱ metabolite from GK. Conclusion: The major metabolic pathways of GA in human are hydroxylation and lactone ring hydrolysis
then hydrolyzation is followed by conjugation with sulfuric acid.The major metabolic pathways of GB are hydroxylation and lactone ring hydrolysis
then hydrolyzation is followed by conjugation with sulfuric acid
glucuronic acid
and glutathione
respectively.The major metabolic pathways of GK is methyl glucuronic acid binding reaction.
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Related Author
CARTIERA Kurban
WANG Changhong
XU Nan
XU Qinwei
TENG Liang
GAO Huijing
Yi-ting GUO
Yao CHENG
Related Institution
State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, The First Affiliated Hospital of Xinjiang Medical University
Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine