Objective: To observe the effect of Huangqi Liuyi Tang on diabetic rats' blood glucose

glycosylated hemoglobin

blood lipid and the renal tubular epithelial reabsorption of glucose transporter 2(SGLT2)

and explore its efficacy and possible mechanismt in the treatment of diabetes. Method: The 77 SPF rats were randomly divided into 6 groups by weight: normal control group

model group

metformin group (0.15 g·kg-1)

Huangqi Liuyi Tang low

middle and high dose groups (3.15

6.30，12.60 g·kg-1). Diabetes models were established by using high fat and high glucose diet for 28 days and then with intraperitoneal injection of streptozotocin at the dose of 40 mg·kg-1 body weight. 7 days after STZ injection

corresponding drugs were given by gavage. Equivalent volume of water was given in the normal control group and model control group by the same method. Gavage medication lasted for six weeks

once a week. Venous blood from the tails was collected to measure blood glycose after 6-hour fasting; after fasting 12-hour at the last time of gavage

the venous blood from the tails was taken for blood glycose and then the rats were anaesthetized to collect the abdominal venous blood. The kidney and pancreas were taken for histopathological examination after the rats were sacrificed. The blood glucose

glycosylated hemoglobin

blood lipid

body weight

food intake

and the expression of renal tissue SGLT2 were observed. Result: The blood glucose levels in modeling groups were higher than those in normal control group (P<0.01)

with a blood glucose value>13.0 mmol·L-1. After six weeks self-healing

the blood glucose level in model group was still significantly higher than that of normal control group (P<0.01); the blood glucose levels in metformin group

Huangqi Liuyi Tang in middle and high dose groups were lower than that in model group (P<0.05). Glycosylated hemoglobin level was increased in model group and higher than that in the normal control group (P<0.01). As compared with the model group

glycosylated hemoglobin level was decreased in metformin group and the Huangqi Liuyi Tang high dose group (P<0.05). In the pancreas pathological HE staining in model rats

complete pancreas islet structure was seen

indicating that islet was not destroyed completely in this experiment. Renal pathological slices showed that the structures of renal glomerulus

collections

tubular

proximal and distal tubule were complete in all groups

with no obvious pathological damages. After treatment

SGLT2 protein expression levels were reduced in various treatment groups (P<0.05

P<0.01). Conclusion: High sugar and high fat diet with intraperitoneal injection of STZ increased SGLT2 protein expression in kidney tissues of diabetic models. Huangqi Liuyi Tang can reduce blood glucose

glycosylated hemoglobin

and lower SGLT2 protein expression in diabetes model rats. Its treatment efficacy for diabetes may be associated with partial inhibition of renal tubular epithelial reabsorption of glucose.